Polycyclic Aromatic Hydrocarbon (PAH) Analysis

Wakopak^® Wakosil-PAHs (LC Column)

Wakopak^® Wakosil-PAHs is a dedicated column that uses packing agents optimized for the analysis of polycyclic aromatics. It is based on high purity silica gel with an average particle size of 5 μm. Shape recognition ability for PAHs is high, and theoretical plate number and resolution are excellent, so a sharp peak can be obtained.

• Standard Mixture Solution (15 PHAs)

  

• Outdoor Air Sample Analysis Application Examples

  

• Column	：Wakopak® Wakosil®-PAHs (4.6 mm x 250 mm)
  Eluent	：A) CH3OH/H2O = 80/20 (v/v) 　B) CH3CN
  Gradient	：0 - 15 min. B conc. 10 % 　5 - 15 min. B conc. 10 → 75 % 　15 - 25 min. B conc. 75 %
  Mixer Volume	：500 µL High Press. Gradient mode

• Flow Rate	：1.0 mL/min. at 30 ℃
  Detection	：FL	0.0min. λ ex 280 nm　λ em 330 nm 6.0min. λ ex 260 nm　λ em 340 nm 8.1min. λ ex 250 nm　λ em 420 nm 12.0min. λ ex 270 nm　λ em 400 nm 17.5min. λ ex 295 nm　λ em 410 nm 19.1min. λ ex 300 nm　λ em 500 nm
  Injection Volume	：10 µL

Sample：	1) Naphthalene	0.5 µg		6) Fluoranthene	0.48 µg		11) Benzo[k]fluoranthene	0.05 µg
	2) Acenaphthene	0.2 µg		7) Pyrene	0.3 µg		12) Benzo[a]pyrene	0.03 µg
	3) Fluorene	0.14 µg		8) Benzo[a]anthracene	0.06 µg		13) Dibenzo[a, h]anthracene	0.08 µg
	4) Phenanthrene	0.25 µg		9) Chrysene	0.2 µg		14) Benzo[g, h, i]perylene	0.06 µg
	5) Anthracene	0.03 µg		10) Benzo[b]fluoranthene	0.3 µg		15) Indeno[1, 2, 3-cd]pyrene	0.4 µg
							in 1 mL CH3CN