Amino Acids Analysis (Analytical Standards and Standard Solutions)

Amino Acids Mixture Standard Solutions (CRM)*

• CRM with assured metrological traceability (SI traceable).
• Uncertainty obtained from stability, homogeneity is assigned.
• The certificate that describes impurities of amino acids is attached.
• Use of "SI traceable reference materials" as raw materials.
• Lineup of five products with different compositions.

Amino Acids Mixture Standard Solutions [High Range Type (non-CRM)]*

• Amino acids mixture standard solution at high concentration than CRM.
• Prepared using high-precision liquid preparation technology cultivated through the development of CRM
• Use "analytical standard with assured amino acids as an impurity" or "reference material with assured SI traceability" as raw materials
• Lineup of three products with different compositions

Product grade	CRM	High Range Type (non-CRM)
Accreditation system	ASNITE	-
Metrological reference	-	-
MRA compatible	✓	-
Certificate	IAJapan certificate	-
SI traceability	✓	-

* 2-Aminoadipic acid is not contained.

Mixture Internal Standard Solutions

• Mixture solution of 24 type labeled amino acids
• Errors caused by "pretreatment", "injection", "ionization efficiency", etc. in LC/MS analysis can be corrected.

This product uses NMIJ CRM or TRM (SI Traceable Reference Material) as raw materials.. Fujifilm Wako has established a reference material production system based on ISO 17034 by transferring accurate liquid preparation technology from National Metrology Institute of Japan (NMIJ), obtaining stability data and concentration confirmation tests at Fujifilm Wako. Using the amino acids mixture standard solution (CRM) assures a reliable amino acid measurement.

*Certificates with logos are only available in Japanese.

Composition

*1 CRM: Certified reference material
*2 Solution 1 and solution 2 are a set of products.

Abbreviation

Abbreviation	Amino acids	Abbreviation	Amino acids	Abbreviation	Amino acids
P-Ser	O-Phospho-L-serine	Ala	L-Alanine	MEA	2-Aminoethanol
Tau	Taurine	Cit	L-Citrulline	NH4	Ammonium Ion
PEA	O-Phosphoethanolamine	Abu	DL-2-Aminobutyric Acid	5Hyl	5-Hydroxy-DL-lysine
Urea	Urea	Val	L-Valine	Orn	L-Ornithine
Asp	L-Aspartic Acid	(Cys)2	L-Cystine	Lys	L-Lysine
Hyp	L-Hydroxyproline	Met	L-Methionine	1Mehis	1-Methyl-L-histidine
Thr	L-Threonine	Hcy(Ala)	L-Cystathionine	His	L-Histidine
Ser	L-Serine	Ile	L-Isoleucine	3Mehis	3-Methyl-L-histidine
Asn	L-Asparagine	Leu	L-Leucine	Ans	L-Anserine
Glu	L-Glutamic Acid	Tyr	L-Tyrosine	Car	L-Carnosine
Gln	L-Glutamine	Phe	L-Phenylalanine	Arg	L-Arginine
Sar	Sarcosine	bAla	β-Alanine
Aad	2-Aminoadipic Acid	bAib	DL-3-Aminoisobutyric Acid
Pro	L-Proline	4Abu	4-Aminobutyric Acid
Gly	Glycine	Trp	L-Tryptophan

Type AN

Components	Concentration (μmol/mL)		Components	Concentration (μmol/mL)
	CRM	High Range Type (non-CRM)		CRM	High Range Type (non-CRM)
L-Aspartic Acid	0.2250-0.2750	2.38-2.62	Taurine	0.1125-0.1375	1.19-1.31
DL-3-Aminoisobutyric Acid	0.2250-0.2750	2.38-2.62	L-Tyrosine	0.2250-0.2750	2.38-2.62
DL-2-Aminobutyric Acid	0.1125-0.1375	1.19-1.31	L-Threonine	0.2250-0.2750	2.38-2.62
β-Alanine	0.2250-0.2750	2.38-2.62	Urea	4.500-5.500	47.5-52.5
L-Alanine	0.2250-0.2750	2.38-2.62	L-Valine	0.2250-0.2750	2.38-2.62
L-Isoleucine	0.2250-0.2750	2.38-2.62	L-Hydroxyproline	0.2250-0.2750	2.38-2.62
Glycine	0.2250-0.2750	2.38-2.62	L-Phenylalanine	0.2250-0.2750	2.38-2.62
L-Glutamic Acid	0.2250-0.2750	2.38-2.62	L-Proline	0.2250-0.2750	2.38-2.62
Sarcosine	0.5625-0.6875	5.94-6.56	O-Phosphoethanolamin	0.1125-0.1375	1.19-1.31
L-Cystathionine	0.1125-0.1375	1.19-1.31	O-Phospho-L-serine	0.1125-0.1375	1.19-1.31
L-Cystine	0.1125-0.1375	2.38-2.62	L-Methionine	0.2250-0.2750	2.38-2.62
L-Citrulline	0.2250-0.2750	2.38-2.62	L-Leucine	0.2250-0.2750	2.38-2.62
L-Serine	0.2250-0.2750	2.38-2.62

Type B

Components	Concentration (μmol/mL)		Components	Concentration (μmol/mL)
	CRM	High Range Type (non-CRM)		CRM	High Range Type (non-CRM)
2-Aminoethanol	0.2250-0.2750	2.38-2.62	L-Carnosine	0.2250-0.2750	2.38-2.62
4-Aminobutyric Acid	0.2250-0.2750	2.38-2.62	L-Histidine	0.2250-0.2750	2.38-2.62
L-Arginine	0.2250-0.2750	2.38-2.62	5-Hydroxy-DL-lysine	0.2250-0.2750	2.38-2.62
L-Anserine	0.2250-0.2750	2.38-2.62	1-Methyl-L-histidine	0.2250-0.2750	2.38-2.62
Ammonium Chloride	0.2250-0.2750	2.38-2.62	3-Methyl-L-histidine	0.2250-0.2750	2.38-2.62
L-Ornithine	0.2250-0.2750	2.38-2.62	L-Lysine	0.2250-0.2750	2.38-2.62

Type H

Components	Concentration (μmol/mL)		Components	Concentration (μmol/mL)
	CRM	High Range Type (non-CRM)		CRM	High Range Type (non-CRM)
L-Aspartic Acid	0.2250-0.2750	2.38-2.62	L-Tyrosine	0.2250-0.2750	2.38-2.62
L-Alanine	0.2250-0.2750	2.38-2.62	L-Threonine	0.2250-0.2750	2.38-2.62
L-Arginine	0.2250-0.2750	2.38-2.62	L-Valine	0.2250-0.2750	2.38-2.62
L-Isoleucine	0.2250-0.2750	2.38-2.62	L-Histidine	0.2250-0.2750	2.38-2.62
Ammonium Chloride	0.2250-0.2750	2.38-2.62	L-Phenylalanine	0.2250-0.2750	2.38-2.62
Glycine	0.2250-0.2750	2.38-2.62	L-Proline	0.2250-0.2750	2.38-2.62
L-Glutamic Acid	0.2250-0.2750	2.38-2.62	L-Methionine	0.2250-0.2750	2.38-2.62
L-Cystine	0.1125-0.1375	2.38-2.62	L-Lysine	0.2250-0.2750	2.38-2.62
L-Serine	0.2250-0.2750	2.38-2.62	L-Leucine	0.2250-0.2750	2.38-2.62

APDSTAG^® Wako Amino Acids Mixture Standard Solution No. 1 [CRM]

Components	Concentration (μmol/mL)	Components	Concentration (μmol/mL)
L-Aspartic Acid	0.1800-0.2200	Sarcosine	0.1800-0.2200
DL-3-Aminoisobutyric Acid	0.1800-0.2200	L-Cystine	0.900-1.100
2-Aminoethanol	0.1800-0.2200	L-Citrulline	0.900-1.100
DL-2-Aminobutyric Acid	0.4500-0.5500	L-Hydroxyproline	0.1800-0.2200
4-Aminobutyric Acid	0.1800-0.2200	5-Hydroxy-DL-lysine	0.1800-0.2200
L-Anserine	0.1800-0.2200	L-Methionine	0.900-1.100
L-Carnosine	0.1800-0.2200	1-Methyl-L-histidine	0.1800-0.2200
L-Glutamic Acid	0.900-1.100	3-Methyl-L-histidine	0.1800-0.2200

APDSTAG^® Wako Amino Acids Mixture Standard Solution No. 2 [CRM]

Components	Concentration (μmol/mL)	Components	Concentration (μmol/mL)
L-Alanine	9.00-11.00	L-Threonine	4.500-5.500
L-Arginine	2.250-2.750	L-Valine	9.00-11.00
L-Isoleucine	4.500-5.500	L-Histidine	4.500-5.500
L-Ornithine	2.250-2.750	L-Phenylalanine	4.500-5.500
Glycine	9.00-11.00	L-Proline	4.500-5.500
L-Serine	4.500-5.500	L-Lysine	4.500-5.500
Taurine	4.500-5.500	L-Leucine	4.500-5.500
L-Tyrosine	4.500-5.500

APDSTAG^® Wako Amino Acids Internal Standard Mixture Solution

Components	Concentration (μmol/L)	Components	Concentration (μmol/L)	Components	Concentration (μmol/L)
L-Asparagine-13C4, 15N2	99	L-Glutamic Acid-13C5, 15N	110	L-Valine-13C5, 15N	55
L-Aspartic Acid-2,3,3-d3	110	L-Cystine-3,3,3’,3’-d4	220	L-Histidine-13C6, 15N3	110
2-Aminoethanol-1,1,2,2-d4	110	L-Citrulline-4,4,5,5-d4	110	L-Phenylalanine-13C9, 15N	88
L-Alanine-13C3	110	L-Threonine-13C4	110	L-Proline-13C5, 15N	27.5
L-Arginine-15N4	110	L-Serine-13C3, 15N	110	L-Methionine-13C5, 15N	55
L-Isoleucine-13C6, 15N	88	Taurine-13C2	275	3-Methyl-d3-L-histidine	100
L-Ornithine-13C5	88	L-Tyrosine-ring-13C6	110	L-Lysine-13C6, 15N2	143
Glycine-13C2,15N	55	L-Tryptophan-13C11, 15N2	88	L-Leucine-5,5,5-d3	88

The concentrations of the Amino Acids Mixture Standard Solution (non-CRM, High Range Type) and conventional product (non-CRM, High Range Type) were determined using the amino acids mixture standard solution (CRM), and the differences between the concentration values were evaluated. The results are shown below.

Conventional product	AN-2: 011-14463, 015-14461	Type B: 012-08643, 016-08641	Type H: 019-08393, 013-08391
This product [Amino Acids Mixture Standard Solution (High Range Type)]	Type AN: 016-28161, 010-28164	Type B: 012-28141, 016-28144	Type H: 019-28151, 013-28154

Type AN

Amino acids	Concentration (μmol/mL)	Concentration divergence between conventional products and this product	Amino acids	Concentration (μmol/mL)	Concentration divergence between conventional products and this product
P-Ser	1.25	-	Val	2.50	-
Tau	1.25	-	(Cys)2	2.50	-
PEA	1.25	max. 5%, min. 2%	Met	2.50	-
Urea	50.0	max. 5%, min. 2%	Hcy(Ala)	1.25	-
Asp	2.50	-	Ile	2.50	-
Thr	2.50	max. 5%, min. 2%	Leu	2.50	-
Ser	2.50	-	Tyr	2.50	-
Glu	2.50	-	Phe	2.50	-
Sar	6.25	max. 5%, min. 2%	bAla	2.50	max. 5%, min. 2%
Gly	2.50	-	bAib	2.50	min. 5%
Ala	2.50	-	Hyp	2.50	max. 5%, min. 2%
Cit	2.50	-	Pro	2.50	-
Abu	1.25	max. 5%, min. 2%

• Type B

  Amino acids	Concentration (μmol/mL)	Concentration divergence between conventional products and this product
  4Abu	2.50	-
  MEA	2.50	-
  NH4	2.50	-
  5Hyl	2.50	-
  Orn	2.50	-
  Lys	2.50	-
  1Mehis	2.50	-
  His	2.50	-
  3Mehis	2.50	-
  Ans	2.50	max. 5%, min. 2%
  Car	2.50	max. 5%, min. 2%
  Arg	2.50	-

  -: No significant difference in concentration value has been observed.

• Type H

  Amino acids	Concentration (μmol/mL)	Concentration divergence between conventional products and this product
  Asp	2.50	-
  Thr	2.50	-
  Ser	2.50	-
  Glu	2.50	-
  Gly	2.50	-
  Ala	2.50	-
  Val	2.50	-
  (Cys)2	2.50	-
  Met	2.50	-
  Ile	2.50	-
  Leu	2.50	-
  Tyr	2.50	-
  Phe	2.50	-
  NH4	2.50	-
  Lys	2.50	-
  His	2.50	-
  Arg	2.50	-
  Pro	2.50	-