Adiponectin ELISA Kits
Adiponectin is a cytokine secreted by adipocytes and plays a role in regulating lipid metabolism and insulin sensitivity and is known for its antidiabetic, anti-atherosclerotic, and anti-inflammatory effects. In the bloodstream, adiponectin exists in multimeric forms: trimers (low molecular weight, LMW), hexamers (medium molecular weight, MMW), and higher-order complexes of 12 to 18 monomers (high molecular weight, HMW). Circulating HMW adiponectin levels, rather than total adiponectin, are considered a more accurate indicator of physiological parameters such as BMI, sex, weight loss, glucose tolerance, hepatic insulin sensitivity, and the presence of metabolic syndrome or type 2 diabetes.
The LBIS™ Mouse/Rat High Molecular Weight Adiponectin ELISA Kit is a sandwich ELISA kit designed for the quantitative determination of HMW adiponectin in mouse and rat samples.
What is Adiponectin?
Adiponectin is a cytokine secreted by adipocytes and classified as an adipocytokine (also referred to as an adipokine). It plays a role in regulating lipid metabolism and insulin sensitivity and is known for its antidiabetic, anti-atherosclerotic, and anti-inflammatory effects. Adiponectin also binds various growth factors and modulates processes such as cell proliferation, angiogenesis, and tissue remodeling by sequestering these factors.
In the bloodstream, adiponectin exists in multimeric forms: trimers (low molecular weight, LMW), hexamers (medium molecular weight, MMW), and higher-order complexes of 12 to 18 monomers (high molecular weight, HMW). Trimers are formed through non-covalent interactions involving the triple-helical structure in the collagenous domain and hydrophobic interactions in the globular C1q domain. Hexamers and larger complexes are generated through the oligomerization of trimers.
Circulating HMW adiponectin levels, rather than total adiponectin, are considered a more accurate indicator of physiological parameters such as BMI, sex, weight loss, glucose tolerance, hepatic insulin sensitivity, and the presence of metabolic syndrome or type 2 diabetes.
LBIS™ Mouse/Rat High Molecular Weight Adiponectin ELISA Kit
This kit is a sandwich ELISA kit designed for the quantitative determination of HMW adiponectin in mouse and rat samples. All reagents are supplied in liquid form and are ready to use.
Kit Performance
| Analysis sample | Mouse Serum/Plasma Rat Serum/Plasma Culture medium |
|---|---|
| Calibration curve range | 3.13-200 ng/mL |
| Sample volume | 10 μL (diluted 25-50 fold) |
| Measurement duration | Approx. 4 hours |
| Detection method | Colorimetric (Primary : 450 nm / Refference : 620 nm) |
Example of Calibration Curve
Cross-Reactivity with Similar Proteins
Using this product, cross-reactivity was assessed by measuring structurally related proteins at each concentrations.
| Species | Similar Proteins | Concentrations (ng/mL) |
Measurement value (ng/mL) |
Cross-Reactivity (％) |
|---|---|---|---|---|
| Mouse | Adiponectin (HMW) | 1,000 | 1,000 | 100 |
| Adiponectin (Hexamer) | 1,000 | <50 | <5 | |
| Adiponectin (Trimer) | 1,000 | N.D. | - | |
| Adiponectin (Monomer) | 1,000 | N.D. | - | |
| MCH | 1,000 | N.D. | - | |
| TNF-α | 1,000 | N.D. | - | |
| INF-γ | 1,000 | N.D. | - | |
| Insulin | 1,000 | N.D. | - | |
| Leptin | 1,000 | N.D. | - | |
| Rat | Adiponectin (HMW) | 1,000 | 1,000 | 100 |
| Adiponectin (Monomer) | 1,000 | N.D. | - | |
| TNF-α | 1,000 | N.D. | - | |
| INF-γ | 1,000 | N.D. | - | |
| Insulin | 1,000 | N.D. | - |
Measurement Using Actual Samples
Adiponectin in serum from mouse/rat
Examples of serum adiponectin measurements in non-fasted mice and rats are shown below.
| Species | Strains | Age (week) |
Sex | Number of samples |
Measurement value (ng/mL) |
SD |
|---|---|---|---|---|---|---|
| Mouse | BALB/c | 6 | Male | 10 | 2,369 | 743 |
| ICR | 6 | Male | 10 | 2,119 | 802 | |
| BKS.Cg-+Leprdb/+Leprdb/ | 12 | Male | 10 | 1,802 | 875 | |
| KKAy/Ta | 12 | Male | 16 | 2,141 | 937 | |
| B6.V-Kepob | 12 | Male | 10 | 1,229 | 437 | |
| Rat | CD (SD) | 8 | Male | 9 | 3,220 | 670 |
| GK | 12 | Male | 10 | 3,512 | 304 | |
| ZUC-Leprfa | 8 | Male | 10 | 1,991 | 1,120 |
- Sample:
- Serum
- Blood Collection Conditions:
- Fasting: None (non-fasted)
Anesthesia: Isoflurane
Sampling site: Heart (abdominal aorta for KKAy/Ta mice) - Housing Conditions:
- KKAy/Ta mice: Housed individually
HMW Adiponectin in male and female mice
Examples of serum HMW adiponectin measurements in male and female mice are shown below.
- Sample:
- Serum
- Blood Collection Conditions:
- Fasting: 24 hours
Anesthesia: Isoflurane
Sampling site: Heart - Food
- Normal Food (CRF-1)
- Housing Conditions:
- Group-Housing
Changes in HMW Adiponectin Following Pioglitazone Administration
Pioglitazone is known to improve insulin resistance by increasing adiponectin expression. The figure shows HMW adiponectin levels in mice before and after pioglitazone administration, as well as in saline-treated control mice.
< Drug administration >
- Species:
- Mouse (KKAy/Ta, Male, 7-weeks old)
- Food:
- CE-2 supplemented with 20% soybean oil
- Before administration:
- (n=12)
- Control:
- 10 mL/kg Saline (n=6)
- Test:
- 10 mg/10 mL/kg Pioglitazone (n=6)
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Adiponectin (High Molecular Weight) ELISA Kit (Mouse/Rat)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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