GLP-1 ELISA Kits
GLP-1 is the gastrointestinal peptide hormone secreted from intestinal L cells, and shows the hypoglycemic action through the enhancement of insulin secretion and the suppression of glucagon secretion by the sugar stimulus. The report has been issued that GLP-1 is useful for the treatment of type 2 diabetes as the new therapeutic agent for diabetes.
GLP-1 ELISA Kit Wako, High Sensitive is ELISA kit measurable with a high sensitivity of GLP-1 in the plasma of mouse and rat used with a GLP-1 antibody. Since this kit reacts to both the active form of GLP-1 (7-36 amide) and the inactive form of GLP-1 (9-36 amide), it is suitable for experimental system measuring the total amount of GLP-1.
What is GLP-1?
An important regulator of blood glucose is glucagon-like peptide-1 (GLP-1), an incretin (a hormone that stimulates insulin secretion) secreted by the L cells of the small intestine in response to the postprandial increase in blood glucose levels. Acting through G protein-coupled receptors highly expressed in pancreatic β cells, GLP-1 enhances insulin secretion1). To date, a number of GLP-1 receptor agonists have been developed for the treatment of diabetes and obesity. Currently, glucagon receptor antagonists, antibodies, and agonists are being investigated as glucagon-related therapies2).
Because GLP-1 secretion is influenced by factors such as diet, the intestinal environment, and short-chain fatty acids (SCFAs) produced by the gut microbiota3), GLP-1 has attracted considerable attention as a target for the prevention and treatment of metabolic diseases.
GLP-1 ELISA Kit Wako, High Sensitive
This kit is an ELISA kit that enables highly sensitive measurement of GLP-1 in mouse and rat plasma using a GLP-1 antibody.
Kit Performance
| Analysis sample | Mouse Plasma (EDTA) Rat Plasma (EDTA) |
|---|---|
| Calibration curve range | 0.94-30 pM |
| Sample volume | 10 μL |
| Measurement duration | Approx. 4 hours |
| Detection method | Colorimetric (Primary: 450 nm / Reference: 620 nm) |
Example of Calibration Curve
Cross-Reactivity with Similar Proteins
Using this product, cross-reactivity was assessed by measuring structurally related proteins at each concentrations.
| Species | Similar proteins | Concentrations (pg/mL) |
Measurement value (pg/mL) |
Cross-Reactivity (%) |
|---|---|---|---|---|
| Mouse/ Rat | GLP-2 | 1,000 | N.D. | - |
| Glucagon (1-29) | 1,000 | N.D. | - | |
| Insulin | 1,000 | N.D. | - | |
| Secretin | 1,000 | N.D. | - | |
| GIP | 1,000 | N.D. | - | |
| VIP | 1,000 | N.D. | - | |
| GRF | 1,000 | N.D. | - | |
| Bovine | Glucagon (1-29) | 1,000 | N.D. | - |
| VIP | 1,000 | N.D. | - | |
| Pig | Glucagon (1-29) | 1,000 | N.D. | - |
| VIP | 1,000 | N.D. | - |
Correlation with products from other competitors
Measurements were conducted on the same plasma samples (n=10) from mouse using this kit and competitor's product M. The measurement values obtained using both products were then compared.
GLP-1 ELISA Kit Wako, High Sensitive (This kit)
| Sample ID | Measurement value (pM) |
mean | SD | CV (%) |
|---|---|---|---|---|
| 1 | 30.7 | 31.4 | 0.976 | 3.1 |
| 32.1 | ||||
| 2 | 16.0 | 16.3 | 0.474 | 2.9 |
| 16.6 | ||||
| 3 | 16.8 | 17.2 | 0.640 | 3.7 |
| 17.7 | ||||
| 4 | 21.3 | 20.8 | 0.651 | 3.1 |
| 20.4 | ||||
| 5 | 27.2 | 27.1 | 0.0884 | 0.3 |
| 27.1 | ||||
| 6 | 40.3 | 39.6 | 1.02 | 2.6 |
| 38.9 | ||||
| 7 | 15.5 | 15.9 | 0.693 | 4.3 |
| 16.4 | ||||
| 8 | 9.18 | 9.43 | 0.354 | 3.8 |
| 9.68 | ||||
| 9 | 7.84 | 8.09 | 0.357 | 4.4 |
| 8.35 | ||||
| 10 | 14.1 | 13.9 | 0.247 | 1.8 |
| 13.7 |
Competitor’s product M
| Sample ID | Measurement value (pM) |
mean | SD | CV (%) |
|---|---|---|---|---|
| 1 | 24.4 | 23.4 | 1.38 | 5.9 |
| 22.4 | ||||
| 2 | 11.9 | 11.4 | 0.665 | 5.8 |
| 10.9 | ||||
| 3 | 13.5 | 14.0 | 0.786 | 5.6 |
| 14.6 | ||||
| 4 | 17.2 | 16.9 | 0.448 | 2.7 |
| 16.5 | ||||
| 5 | 20.7 | 20.5 | 0.284 | 1.4 |
| 20.3 | ||||
| 6 | 20.1 | 19.5 | 0.719 | 3.7 |
| 19.0 | ||||
| 7 | 11.6 | 11.2 | 0.668 | 6.0 |
| 10.7 | ||||
| 8 | 7.28 | 8.87 | 2.25 | 25.4 |
| 10.5 | ||||
| 9 | 9.50 | 8.64 | 1.22 | 14.1 |
| 7.78 | ||||
| 10 | 8.02 | 8.27 | 0.350 | 4.2 |
| 8.52 |
[Result]
Although the results obtained with this kit showed a strong correlation with those obtained with Competitor Product M, discrepancies in the measurement values were observed at high concentrations. Furthermore, the CV for this kit was below 5%, whereas a higher degree of variability was observed with Competitor Product M.
Examples of Measurement Using Actual Samples
Plasma samples from mouse were measured.
| Mouse No. | Measured value (pM) |
CV (%) | Remarks |
|---|---|---|---|
| 1 | 31.4 | 3.1 | Fasted mouse, 8-week-old ♂ ※ Collected the blood after 15 hours fast |
| 2 | 16.3 | 2.9 | |
| 3 | 17.2 | 3.7 | |
| 4 | 20.8 | 3.1 | |
| 5 | 27.1 | 0.3 | |
| 6 | 39.6 | 2.6 | Sugar-administered mouse, 8-week-old ♂ ※ After the fast, administered glucose (2g / kg), then 10 minutes later, collected the blood. |
| 7 | 15.9 | 4.3 | |
| 8 | 9.4 | 3.8 | |
| 9 | 8.09 | 4.4 | |
| 10 | 13.9 | 1.8 |
References
- Perley, M. J. & Kipnis, D. M. : J Clin Invest., 46 (12), 1954 (1967).
Plasma Insulin Responses to Oral and Intravenous Glucose: Studies in Normal and Diabetic Subjects* - Jia, Y. et al . : Front Endocrinol (Lausanne), 13, 928016 (2022).
Role of Glucagon and Its Receptor in the Pathogenesis of Diabetes - Tolhurst, G., Heffron, H., Lam, Y.S. et al.: Diabetes, 364 (2), 61 (2012)
Short-chain fatty acids stimulate glucagon-like peptide-1 secretion via the G-protein-coupled receptor FFAR2.
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Total GLP-1 ELISA Kit
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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