Presep® PolyChelate

SPE Columns for Metal (Elemental) Capture and Recovery "Presep® PolyChelate"

Fujifilm Wako offers the Presep® series which is solid-phase extraction columns packed with a variety of packing agents for use in the pretreatment of food, environmental, and pharmaceutical component analysis.
Presep® PolyChelates are syringe-type columns for metal (elemental) capture and recovery, filled with a packing agent with a long-chain aminocarboxylic acid group as the chelating functional group. Compared to general SPE column, filled IDA (iminodiacetic acid) chelating resins, Presep® PolyChelate can capture and recover many elements (metals) with higher efficiency over a wide pH range. It can also efficiently capture and recover metal oxoacids (Mo, V, W) under acidic to neutral conditions without recovering alkali metals or alkaline earth metals. Presep® PolyChelate can be used for extraction and concentration of trace metals in seawater, river water, wastewater, and biological samples, as well as for removal of matrix in measurements by atomic absorption and ICP emission spectrometry.

Features

  • Capable of capturing and recovering many metals (elements) with high efficiency over a wide pH range!
  • Efficient capture and recovery of metal oxoacids (Mo, V, W) under acidic to neutral conditions without recovery of alkali metals and alkaline earth metals!
  • Can be used as a SPE column in the separation and concentration method by chelating resin in JIS K 0102 "testing methods for industrial wastewater!"

Correlation Between pH and Recovery of 62 Elements When Using Presep® PolyChelate1)

00995_img01R.png
[Instrument]
PerkinElmer Optima 3000 DV ICP-AES, cross-flow nebulizer and a Scott-type spray chamber
1) Provided by Prof. Kagaya, Faculty of Engineering, University of Toyama

Example of Solid-phase Extraction Operation

Example of solid-phase extraction operation*

Column Conditioning

  1. CH3OH5 mL
  2. 3 mol/L HNO310 mL
  3. H2O20 mL
  4. 0.1 mol/L CH3COONH410 mL

pH Adjustment of the Sample

  1. 0.1 mol/L CH3COONH45 mL/100 mL
  2. pH Adjust using HNO3 or NH3 solution.

Loading*1

  1. Sample~500 mL
  2. H2O
    or
    0.1 mol/L CH3COONH4 (pH5~6)*210 mL

Flow rate: 3~10 mL/min.

*1 For samples with high solid content, perform solid-phase extraction after filtration through an appropriate filter.
*2 Used to remove alkaline earth metal elements from samples captured under alkaline conditions.

Elution

  1. 1~3 mol/L HNO33~5 mL

Analysis

* These conditions are only example and may require further study depending on the sample.

Instruction for use and handling

  1. Products must be conditioned prior to use. Drying the packing agent during conditioning may result in loss of reproducibility.
  2. Add a buffer solution that does not interact with elements (metals) such as ammonium acetate before pH adjustment of the sample solution.
  3. When passing the sample solution, reduce or increase the pressure to maintain a constant flow rate.
  4. To avoid adsorption and elution of elements (metals), please refrain from using metal utensils. Use glass or plastic utensils that have been thoroughly rinsed with nitric acid.

Example of Measurement of Elements in Treated Wastewater and Surface Seawater

Solid-phase extraction operation2)

Column Conditioning

  1. CH3OH5 mL
  2. 3 mol/L HNO310 mL
  3. H2O20 mL
  4. 0.1 mol/L CH3COONH410 mL

pH Adjustment of the Sample

  1. 0.1 mol/L CH3COONH45 mL/100 mL
  2. pH Adjust using HNO3 or NH3 solution.

Loading*1

  1. Sample~500 mL
  2. H2O

Flow rate: 3~10 mL/min.

Elution

  1. 1~3 mol/L HNO33~5 mL

Analysis

Recovery rate2)

✔ Trace metal oxoacids (Mo, V) in seawater containing a large amount of alkali metals can be captured and recovered efficiently!

Elements Treated wastewater*1 (n=3) Surface seawater*2 (n=3)
Detection (μg/L) Recovery rate*3 (%) Detection (μg/L) Recoveryrate*3 (%)
Cd 0.2±0.02 93±2.9 (0.03±0.004) 96±1.7
Co <0.033 92±3.7 (0.02±0.012) 96±1.5
Cu 1.1±0.13 104±3.5 0.77±0.017 112±3.2
Fe 33±3.3 114±13.8 1.3±0.05 94±3.2
Mn 871±75 64±9.0 Not measure Not measure
Mo 458±9.3 98±1.7 7.4±0.07 100±4.5
Ni 11±0.2 101±4.9 0.46±0.017 100±1.8
Pb <0.22 94±2.7 0.09±0.052 104±2.0
V <0.22 92±3.1 1.3±0.05 96±2.5
Zn 11±3.1 83±2.1 2.5±0.24 102±2.2

*1 Sample volume: 200 mL (pH 5.5)
*2 Sample volume: 300 mL (pH 4.0)
*3 Internal standard solution (5 μg of each element) added to the solution

2) Kagaya, S., Maeba, E., Inoue, Y., Kamichatani, W., Kajiwara, T., Yanai, H., Saito, M. and Tohda, K.: Talanta., 79, 146 (2009).

Product List

  • Open All
  • Close All

Related Product List

  • Open All
  • Close All

Author: Y. Higai

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

The prices are list prices in Japan.Please contact your local distributor for your retail price in your region.

Please contact us via the inquiry form.