Polymer Gel Wakopak® Wakobeads® -G Series
Wakopak® Wakobeads® (WB)-G series is an HPLC column filled with a polyacrylic acid esters porous spherical gel and was designed on the basis of aqueous GPC separation.
This column is designed for aqueous GPC analysis but is packed uniformly in high density with a chemically stable and less swollen / contracted gel. So it can be used in mixture with an organic solvent or 100% organic solvent. The recommended organic solvents are methanol, acetone, chloroform, dioxane, tetrahydrofuran, N,N-dimethylformamide, and dimethyl sulfoxide.
Four types by each exclusion limit of molecular weight are available. Cartridge precolumn WB-F (1 holder + 4.0 mm x 10 mm cartridge) and replacement cartridge column WB-FC (4 pieces per cartridge) are available for protection of the analytical column.
Applications
Calibration Curve
Data
Physical Properties and Size
| Type of Column (Packing Agent) | WB-G-20 | WB-G-30 | WB-G-40 | WB-G-50 |
|---|---|---|---|---|
| Column Size | 7.8 x 300 mm *1 | |||
| Exclusion Limit of Molecular Weight*2 | 6 x 103 | 5 x 104 | 4 x 105 | 2 x 106 |
| Number of Theoretical Plates | 8,000 | 7,000 | 8,000 | 8,000 |
| Upper Limit of Column Pressure | 30 kg/cm2 or less | |||
| Upper Limit of Flow Rate | 1.5 mL/min. | |||
| Optimal Flow Rate | 1.0 mL/min. | |||
Solvent Type / Exclusion Limit
| Type of Column (Packing Agent) | WB-G-20 | WB-G-30 | WB-G-40 | WB-G-50 |
|---|---|---|---|---|
| Water-Pullulan, PEG | 6 x 103 | 5 x 104 | 4 x 105 | 2 x 106 |
| THF-Polystyrene | 2.5 x 103 | 5.5 x 104 | 5.5 x 105 | 2 x 106 |
| CHCl3-Polystyrene | 1 x 103 | 5.5 x 104 | 4 x 105 | 2 x 106 |
| DMF-Polystyrene | 3.5 x 103 | 6 x 104 | 4 x 105 | 2 x 106 |
*1: Column type is Waters type
*2: Measured value with water 100% mobile phase
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