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  5. [NIPPON GENE] Agarose

[NIPPON GENE] Agarose

NIPPON GENE provides agarose which is specially purified for the purpose of separation and recovery of DNA fragments. Sufficient separation and purity of recovered DNA are ensured by strict quality control.

Purpose and specification

Product Name S、S＜Tablet＞ HS L H XP 21 X
Feature/Application Standard Blotting, etc. Recovering DNA High gel strength Recovering DNA, 3 % gel 3% gel 3% gel
Separation range (kbp) 0.5 - 30 0.5 - 30 0.5 - 30 1 - 200 0.01 - 20 0.01 - 1.0 0.01 - 1.0
Concentration range 0.5 - 2% 0.5 - 2% 0.5 - 2% 0.2 - 1% 1 - 4% 2 - 5% 2 - 6%
No weighing required 0.5 g/tablet*1 3g*2
Gel strength ≥1,200g/cm2
(1.5%)		 ≥1,600g/cm2
(1.5%)		 ≥450g/cm2
(1.5%)		 ≥2,600g/cm2
(1.5%)		 ≥450g/cm2
(1.5%)		 ≥800g/cm2
(3%)		 ≥1,200g/cm2
(4%)
Melting point ≤90°C
(1.5%)		 ≤93°C
(1.5%)		 ≤65°C
(1.5%)		 boil
(1.5%)		 ≤65°C
(1.5%)		 ≤85°C
(3%)		 ≤92°C
(4%)
Gelation temperature 37～39°C
(1.5%)		 37～39°C
(1.5%)		 ≤30°C
(1.5%)		 37～39°C
(1.5%)		 ≤30°C
(1.5%)		 34～38°C
(3%)		 31～39°C
(4%)

*1 Agarose S is a tablet containing 0.5 g of Agarose S.
*2 Stick type (3 g) is also available for Agarose 21.

Quality control

  1. Gel strength
  2. Melting point
  3. Gelation temperature
  4. Sulfate content (SO4)
  5. Water content
  6. Electroendosmosis (-Mr)
  7. No detection of DNase and RNase
  8. Check for recovered DNA
    1. Restriction enzyme cleavage
    2. Ligation with T4 DNA ligase
    3. Transformation of E. coli with ligated DNA (e.g., pBR322)

Product List

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Agarose S

Agarose HS

Agarose L

Agarose H

Agarose XP

Agarose 21

Agarose X

Related Product List

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Antifoam Agent for Agarose Gel

Nucleic Acid Extraction/Purification Reagents (PCR Product/Agarose)

Low Mutagenic Nucleic Acid Staining Reagents　SAFELOOK™

Nucleic Acid Staining Reagents (Under Visible Light)

Ethidium Bromide

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

The prices are list prices in Japan.Please contact your local distributor for your retail price in your region.

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