[NIPPON GENE] PCR Reagents

Gene Taq

Gene Taq is a thermostable DNA polymerase ideal for PCR, isolated and purified from a modified DNA polymerase gene of Thermus aquaticus YT1 expressed in E. coli. It also has terminal transferase activity, and the resulting PCR product can be used for TA cloning. It does not have 5'→3' exonuclease activity because the N-terminus of Taq DNA polymerase is partially deleted. In addition, it shows especially high amplification efficiency for DNA fragments of 1 kbp or less.

Features

• Especially high amplification efficiency for DNA fragments of ≤1 kb
• PCR products can be used for TA cloning.

Gene Taq NT

Gene Taq NT is a thermostable DNA polymerase that is isolated and purified from a cloned DNA polymerase gene of Thermus aquaticus YT1 expressed in E. coli. It has the same function as that of native Taq DNA polymerase and can be used for general PCR work. It also has terminal transferase activity, and the resulting PCR product can be used for TA cloning.

Features

• Functionally the same as a native Taq DNA polymerase
• PCR products can be used for TA cloning.

Gene Taq FP

Gene Taq FP is a Taq DNA polymerase further purified from Gene Taq using our proprietary techniques, with minimal contamination by genomic DNA from the host organism. This product has the same function as does Gene Taq, and is characterized by high amplification efficiency for DNA fragments of 1 kbp or less and no 5'→3' exonuclease activity. It also has terminal transferase activity, and the resulting PCR product can be used for TA cloning. It is ideal for applications greatly affected by genomic DNA contamination, such as RAPD PCR.

Features

• Further purified from Gene Taq
• Taq DNA polymerase with minimal contamination by DNA from the host organism
• PCR products can be used for TA cloning

Gene RED PCR Mix Plus

Gene RED PCR Mix Plus is a 2 x premix type PCR reagent. It contains components required for PCR, such as Taq DNA polymerase, dNTPs, and Mg2+, allowing PCR to take place by simply adding template DNA and a primer. As it contains a specific gravity adjuster and dyes, after PCR the reaction solution can be applied directly to an electrophoresis gel. The buffer composition is also optimized to support high-speed PCR. It can amplify relatively GC-rich sequences and long chains (10 kb) and the resulting PCR product can be used for TA cloning, supporting a wide range of experiments.

Features

• 2 x premix type for ease of operation
• High-speed reaction possible (elongation time 10 sec/kb)
• Containing two dyes, allowing direct application
• Available for various samples

Hot-Start Gene Taq

Hot-Start Gene Taq is a thermostable DNA polymerase for hot start PCR. Before entering a PCR cycle, the enzyme is activated by heat treatment at 95°C for 5 minutes. This product is chemically modified from “GeneTaq FP,” a modified Taq DNA polymerase with minimal contamination by DNA from the host organism. The resulting PCR product can be used for TA cloning. This product comes with two reaction buffers, 10 x Gene Taq Universal Buffer, a traditional buffer; and 10 x Brilliant Buffer. 10 x Brilliant Buffer is an effective buffer for amplification of short DNAs (especially 1.5 kb or less) if you want to increase PCR specificity. す。

Features

• High specificity and high DNA yield
• Hot-Start capability allowing preparation of reaction solution at room temperature
• Best for multiplex PCR
• Containing two buffers, either of which can be used, depending on experimental conditions

Hot-Start Gene Taq NT

Hot-Start Gene Taq NT is a heat-resistant DNA polymerase for hot start PCR. Before entering a PCR cycle, the enzyme is activated by heat treatment at 95°C for 5 minutes. This product is chemically modified from “GeneTaq NT,” which has the same function as a native Taq DNA polymerase. It has 5'→3' exonuclease activity but does not have 3'→5' exonuclease activity. It also has terminal transferase activity, and the resulting PCR product can be used for TA cloning.

Features

• High specificity
• Hot-Start type
• PCR products can be used for TA cloning.

Hot-Start Gene RED PCR Mix

Hot-Start Gene RED PCR Mix is a 2 x premix type PCR reagent developed from a conventional product, Gene RED PCR Mix Plus, to enable hot start PCR using the antibody method. It allows PCR to take place by simply adding a template DNA and a primer.

Features

• Premix type reagent with two dyes
• Simple and speedy use
• Hot Start PCR method suppresses nonspecific amplification
• Supports a wide range of experiments while ensuring a sufficient amplification level

Go-to DNA Polymerase

Go-to DNA Polymerase is a PCR product that combines an α-type DNA polymerase from Pyrococcus sp. and a modified extension enhancer. It has 3'→5' exonuclease activity that can remove nucleotides erroneously incorporated during the polymerase reaction, making it ideal for situations that require highly accurate DNA fragments, such as cloning. Furthermore, it includes a modified elongation enhancer to improve the poor elongation speed and amplification efficiency of α-type DNA polymerases. The product is a "highly reliable (Go-to)" PCR enzyme that provides excellent elongation capability while retaining high accuracy.

Features

• Modified elongation enhancer allowing rapid elongation and excellent DNA amplification efficiency
• Has proofreading activity and provides high accuracy

Pho DNA Polymerase

Pho DNA Polymerase is a heat-resistant DNA polymerase from a hyperthermophile, Pyrococcus horikoshii OT3. It does not have 5'→3' exonuclease activity. Due to its 3'→5' exonuclease activity (proofreading activity), the resulting PCR product is blunt-ended. After phosphorylation, if needed, it can be ligated to a blunt-ended vector. In addition, the frequency of mutations is about 10 times lower than that of Pol type I and the fidelity of synthesis is high, making it ideal for PCR requiring accurate amplification.

Features

• Has 3'→5' exonuclease activity (proofreading activity). PCR products are blunt-ended
• High fidelity of synthesis, allowing highly accurate PCR
• Can amplify long DNA or DNA with high GC content
• Less susceptible to SDS, an inhibitor of PCR
• No enzyme inactivation at a PCR denaturation temperature of 92°C to 98°C

dNTPs Mixture (25 mM each) is a mixture of deoxynucleoside triphosphates (dNTPs), each at a concentration of 25 mM. It can be used as a substrate for DNA polymerases.

ISOSPIN PCR Product

ISOSPIN PCR Product is a kit use to purify PCR products in PCR reaction solutions using spin columns. This kit can remove DNA polymerases, salts, primers, dNTPs, etc. from the PCR reaction solution in as little as 20 minutes, to retrieve only PCR products. The PCR product purified by this kit can be used for various molecular biology experiments.

Features

• High operability achieved using the spin columns we have developed
• High-concentration DNA can be retrieved in about 20 minutes
• Allows easy desalting and primer removal
• No use of toxic organic solvents