Glutamate Dehydrogenase (GLDH) Assay Kit
GLDH is an essential enzyme to synthesize urea and it catalyzes the reversible conversion from glutamate to alpha-ketoglutarate.
Clinically GLDH is a deviation enzyme in blood which deviates from cells when the cells are damaged, only low level of GLDH is found in serum from healthy individuals. As GLDH is particularly abundant in hepatocytes, it is used as a marker for liver injury in toxicity studies and related applications.
LabAssay™ GLDH (AA-type) is a research reagent for measuring GLDH for use with automated analyzer in samples. GLDH levels in samples can be measured quickly.
What is Glutamate Dehydrogenase (GLDH)?
Glutamate dehydrogenase (GLDH) exists in most microbes and in mitochondria of eukaryotes. In mammalian, GLDH is found in many tissues including liver, kidney, heart and brain, but especially in hepatocytes. GLDH is an essential enzyme to synthesize urea and it catalyzes the reversible conversion from glutamate to alpha-ketoglutarate.
Clinically GLDH is a deviation enzyme in blood which deviates from cells when the cells are damaged, only low level of GLDH is found in serum from healthy individuals.
In preclinical toxicity test, ALT in blood has been measured as a marker of liver injury, however, ALT does not change in slight liver injury. Also some reports ALT increase by other causes than liver injury. The combination measuring of GLDH and ALT enable to detect liver injury with higher sensitivity as GLDH is specific biomarker of liver injury.
Methods of GLDH Measurement
Principle of the GLDH assay
GLDH in the sample catalyzes the conversion of ammonia and α-ketoglutarate to glutamate. During this reaction, NADH is oxidized to NAD+, resulting in a decrease in absorbance at 340 nm. The GLDH level is determined by measuring this decrease in absorbance.
Figure 1. Principle of the GLDH assay
LabAssay™ GLDH (AA-type)
LabAssay™ GLDH (AA-type) is a research reagent for measuring GLDH for use with automated analyzer in samples. GLDH levels in samples can be measured quickly.
Kit Performance
| Analysis sample | Human Serum/Plasma Mouse Serum/Plasma Rat Serum/Plasma Dog Serum/Plasma Rabbit Serum/Plasma |
|---|---|
| Calibration curve range | 1.0-150 U/L |
| Sample volume | 15 μL (in case of LABOSPECT 003) |
| Measurement duration | Approx. 10 min |
| Wavelength | Primary wavelength 340 nm Reference wavelength 405 nm |
Compatible Automated Analyzers
- LABOSPECT 003 (Hitachi High-Tech) etc.
Please contact us for other compatible automated analyzers, assay parameters, and protocols.
Examples of measurement
| Animal | Sample* | Measured value (U/L) |
|---|---|---|
| Human | Serum | 5.9 |
| Plasma (EDTA) | 1.1 | |
| Mouse (BALB/c, ♂) |
Serum | 6.3 |
| Plasma (EDTA) | 19.0 | |
| Rat (SD) |
Serum | 13.0 |
| Plasma (EDTA) | 2.8 | |
| Dog (Beagle) |
Serum | 9.4 |
| Plasma (EDTA) | 4.1 | |
| Rabbit (Japanese White Rabbit) |
Serum | 11.6 |
| Plasma (EDTA) | 19.6 |
Correlation with products from other manufacturers
GLDH levels in serum and plasma from humans, mice, rats, dogs, and rabbits were measured using LabAssay™ GLDH (AA-type) (hereinafter, this assay kit) and assay reagents from Company A and Company B. The correlations between the measurement results of each product were evaluated.
[Result]
A strong correlation was confirmed between the measurements obtained using this assay kit and other manufacturers’ products.
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LabAssay™ GLDH (AA-type)
Control serum for measurement accuracy control (AA-type)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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