Column for DNA/RNA separation and purification "Presep® DNA/RNA"

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Presep® DNA/RNA columns are solid-phase extraction columns suitable for the separation and purification of nucleic acids. Type A columns are mini-columns filled with a silica-based separating agent in a barrel-shaped syringe, which can be used for solid-phase extraction in reversed-phase systems.

Features

  • Achieves a high sample load​
  • Enables a sample load 3 to 5 times higher than commercially available pretreatment columns
  • Excellent deprotection efficiency
  • Enables purification with a high level of purity and high recovery rate

Product Appearance

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Specification

No. Product Name Packing Materials Syringe Size Purification amount
1. Presep® DNA/RNA Type A (85 mg / 1 mL) Silica gel 5.5 φ × 57 mm 0.2-0.5 μmol
2. Presep® DNA/RNA Type A (255 mg / 3 mL) Silica gel 9.0 φ × 63 mm 1-1.5 μmol
3. Presep® DNA/RNA Type A (1.0 g / 15 mL) Silica gel 15 φ × 87 mm 4-6 μmol
4. Presep® DNA/RNA Type A (1.7 mg / 25 mL) Silica gel 21 φ × 85 mm 6-10 μmol
5. Presep® DNA/RNA Type A (5.1 mg / 70 mL) Silica gel 27 φ × 134 mm 20-30 μmol

Precautions for Use​

Presep® DNA/RNA should be used in the aspiration mode where the exit side of the column is depressurized and aspirated. An aspiration manifold is useful for handling multiple samples.​

Solid-phase extraction conditions (recommended)​

1. Presep® DNA/RNA Type A (85 mg / 1 mL)

Steps Materials Amount Used
Conditioning Acetonitrile 1 mL
100 mg/mL NaCl aq. 1 mL × 2
Sample Load Sample solution 0.5 mL + 100 mg/mL NaCl aq. 0.5 mL 1mL
Washing 1 Acetonitrile/100 mg/mL NaCl aq.=5/95(v/v) 1 mL × 2
Deprotection 2% DCA*1 aq. (DCA/Water=2/98(v/v)) 1 mL
Washing 2 RNase Free Water 1 mL × 2
Elution 0.5% NH4OH in Acetonitrile/Water=50/50(v/v)*2 1 mL

2. Presep® DNA/RNA Type A (255 mg / 3 mL)

Steps Materials Amount Used
Conditioning Acetonitrile 3 mL
100 mg/mL NaCl aq. 3 mL × 2
Sample Load Sample solution 1 mL + 100 mg/mL NaCl aq. 1 mL 2 mL
Washing 1 Acetonitrile/100 mg/mL NaCl aq.=5/95(v/v) 3 mL × 2
Deprotection 2% DCA*1 aq. (DCA/Water=2/98(v/v)) 3 mL
Washing 2 RNase Free Water 3 mL × 2
Elution 0.5% NH4OH in Acetonitrile/Water=50/50(v/v)*2 3 mL

3. Presep® DNA/RNA Type A (1.0 g / 15 mL)

Steps Materials Amount Used
Conditioning Acetonitrile 12 mL
100 mg/mL NaCl aq. 12 mL × 2
Sample Load Sample solution 5 mL + 100 mg/mL NaCl aq. 5 mL 10 mL
Washing 1 Acetonitrile/100 mg/mL NaCl aq.=5/95(v/v) 12 mL × 2
Deprotection 2% DCA*1 aq. (DCA/Water=2/98(v/v)) 12 mL
Washing 2 RNase Free Water 12 mL × 2
Elution 0.5% NH4OH in Acetonitrile/Water=50/50(v/v)*2 12 mL

4. Presep® DNA/RNA Type A (1.7 g / 25 mL)

Steps Materials Amount Used
Conditioning Acetonitrile 20 mL
100 mg/mL NaCl aq. 20 mL × 2
Sample Load Sample solution 10 mL + 100 mg/mL NaCl aq. 10 mL 20 mL
Washing 1 Acetonitrile/100 mg/mL NaCl aq.=5/95(v/v) 20 mL × 2
Deprotection 2% DCA*1 aq. (DCA/Water=2/98(v/v)) 20 mL
Washing 2 RNase Free Water 20 mL × 2
Elution 0.5% NH4OH in Acetonitrile/Water=50/50(v/v)*2 20 mL

5. Presep® DNA/RNA Type A (5.1 mg / 70 mL)

Steps Materials Amount Used
Conditioning Acetonitrile 60 mL
100 mg/mL NaCl aq. 60 mL × 2
Sample Load Sample solution 20 mL + 100 mg/mL NaCl aq. 20 mL 40 mL
Washing 1 Acetonitrile/100 mg/mL NaCl aq.=5/95(v/v) 60 mL × 2
Deprotection 2% DCA*1 aq. (DCA/Water=2/98(v/v)) 60 mL
Washing 2 RNase Free Water 60 mL × 2
Elution 0.5% NH4OH in Acetonitrile/Water=50/50(v/v)*2 60 mL
*1 DCA = Dichloroacetic Acid
*2 Add 50 µL of 28% ammonium hydroxide per 10 mL of 50% acetonitrile in water.

Comparison of separation capacity​

1. Recovery of oligonucleotide​

Sample of Cartridge Column

Wako
(Presep® DNA/RNA Type A)
Competitor A Competitor B Competitor C
Particle Support Silica gel Silica gel Polymer Polymer

Sample of Oligonucleotide

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Sample Name Length Linkage Structure
Sample 1 14 mer PO
Sample 2 21 mer PO
Sample 3 21 mer PS
Sample 4 50 mer PO

Cartridge Column vs Recovery of oligonucleotide [%]

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Presep® DNA/RNA Type A
achieves high recovery.
Wako ≒ B > A > C
  • Oligonucleotide samples were purified using the protocol recommended by each company.​
  • Oligonucleotide concentration was measured with an absorbance meter.​
  • Recovery was calculated as "oligonucleotide concentration in the eluate/oligonucleotide concentration before loading".​

2. Allowable load of oligonucleotide​

Sample Load vs Recovery of oligonucleotide [%]

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Presep® DNA/RNA Type A has
a high support retention capacity.

When the ratio of the load of oligonucleotide to support was increased to 9.7, Wako’s product maintained a recovery rate of 75% or higher in contrast to competitor products (A, B, and C) that had a recovery rate of less than 65%.

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Eluents

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For research use or further manufacturing use only. Not for use in diagnostic procedures.

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