[VitaCyte] Collagenase / Neutral Protease
VitaCyte offers the broadest line of purified-defined cell and tissue dissociation enzymes on the market. Use a Collagenase with a Neutral Protease enzyme or one or both of the PD Collagenase products, that takes the guesswork out of most common tissue dissociation applications.
Overview
| Purified-Defined Collagenase | ||||||
|---|---|---|---|---|---|---|
| Wako Cat. No. |
Catalog ID | Product Name | Package Size | GMP grade |
Animal Origin Free |
Application |
| 554-57281 | 001-1000 | CIzyme Collagenase HA | 2000 Wunsch U | Human Islet | ||
| 551-57291 | 001-1050 | CIzyme Collagenase HA | 200 Wunsch U | Rodent Hepatocytes | ||
| 554-57301 | 001-2030 | CIzyme Collagenase MA | 2.5 mil CDA U | Human Hepatocytes, Pig Islets, Rodent Hepatocytes |
||
| 551-57311 | 001-4010 | rCollagenase HI (recombinant collagenase) |
1600 Wunsch U 1.5 million CDA U |
Human Islet | ||
| 550-57381 | 011-1060 | Collagenase Gold | 1 g | Human Islet | ||
| 557-57391 | 011-2000 | Collagenase Gold Plus | >1500 FALGPA Units | Human Islet | ||
| Purified Neutral Protease | ||||||
|---|---|---|---|---|---|---|
| Wako Cat. No. |
Catalog ID | Product Name | Package Size | GMP grade |
Animal Origin Free |
Application |
| 555-57331 | 003-1000 | CIzyme BP Protease | 1.1 million NP U | Human Hepatocytes, Human Islet, Human Skin, Pig Islets, Rodent Hepatocytes |
||
| 552-57341 | 003-2000 | CIzyme BP Protease | 2.3 million NP U | Human Hepatocytes, Human Islet, Human Skin, Pig Islets, Rodent Hepatocytes |
||
| 558-57321 | 002-3000 | CIzyme Thermolysin | 6 mg | Human Islet | ||
| 559-57351 | 004-2000 | CIzyme Clostripain | 25 mg | Human Islet | ||
| Application Specific Purified-Defined Collagenase Mixture | ||||||
|---|---|---|---|---|---|---|
| Wako Cat. No. |
Catalog ID | Product Name | Package Size | GMP grade |
Animal Origin Free |
Application |
| 556-57361 | 005-1030 | CIzyme RI | 375K CDA U+ 75K NPA U | Rodent Islet | ||
| 553-57371 | 005-1090 | CIzyme AS | 35 Wunsch U+ 280K NPA U | Human Adipose | ||
| Purified-Defined Collagenase & BP Protease Mixture | ||||||
|---|---|---|---|---|---|---|
| Wako Cat. No. |
Catalog ID | Product Name | Package Size | GMP grade |
Animal Origin Free |
Application |
| 550-57401 | 011-3010 | PD Collagenase 100 | 1 g | Pig Hepatocytes, Rodent Hepatocytes, Stem Cell |
||
| 557-57411 | 011-3020 | PD Collagenase 800 | 1 g | Rodent Cardiomyocytes | ||
Purified-Defined Collagenase
CIzyme Collagenase HA (#001-1000)
GMP Grade Collagenase HA is an aseptically prepared, lyophilized mixture of 60% purified Class I (C1) Collagenase and 40% purified Class II (C2) Collagenase from Clostridium histolyticum.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 2000 Wünsch U | Human Islet |
CIzyme Collagenase HA (#001-1050)
GMP Grade Collagenase HA is an aseptically prepared, lyophilized mixture of 60% purified Class I (C1) Collagenase and 40% purified Class II (C2) Collagenase from Clostridium histolyticum.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 200 Wünsch U | Rodent Hepatocytes |
CIzyme Collagenase MA (#001-2030)
GMP Grade Collagenase MA is an aseptically filled, lyophilized protein mixture containing 60% purified Class I (C1) and 40% purified Class II (C2) collagenase from Clostridium histolyticum.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 2.5 Million CDA U | Human Hepatocytes, Rodent Hepatocytes |
rCollagenase HI (recombinant collagenase) (#001-4010)
GMP Grade Animal Origin Free (AOF) rCollagenase HI is an aseptically prepared, lyophilized mixture of purified recombinant collagenase C1 (ColG) and purified recombinant collagenase C2 (ColH) from engineered E. coli.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 1600 Wünsch U | Human Islet |
Collagenase Gold (#011-1060)
Collagenase Gold is low protease, purified defined collagenase product from Clostridium histolyticum that can be used for isolating cells from tissue or recovering adherent cells after in vitro culture.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 1 g | Human Islet |
Collagenase Gold Plus (#011-2000)
GMP Grade Collagenase Gold Plus is an aseptically prepared, lyophilized purified defined collagenase that contains both Class I and Class II Collagenase from Clostridium histolyticum.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| >1,500 FALGPA U | Human Islet |
Purified Neutral Protease
CIzyme BP Protease (#003-1000)
GMP Grade Animal Origin Free (AOF) BP Protease is an affordable, aseptically dispensed, lyophilized protein containing purified neutral protease from Bacillus polymyxa.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 1.1 Million NP U | Human Hepatocytes, Human Islet, Human Skin, Pig Islets, Rodent Hepatocytes |
CIzyme BP Protease (#003-2000)
GMP Grade Animal Origin Free (AOF) BP Protease is an affordable, aseptically dispensed, lyophilized protein containing purified neutral protease from Bacillus polymyxa.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 2.3 Million NP U | Human Hepatocytes, Human Islet, Human Skin, Pig Islets, Rodent Hepatocytes |
CIzyme Thermolysin (#002-3000)
GMP Grade Animal Origin Free (AOF) Thermolysin is an aseptically dispensed, lyophilized purified protease from Bacillus thermoproteolyticus.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 6 mg | Human Islet |
CIzyme Clostripain (#004-2000)
GMP Grade Clostripain is an unactivated enzyme from Clostridium histolyticum that is purified, aseptically filled, and then lyophilized.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 25 mg | Human Islet |
Application Specific Purified-Defined Collagenase Mixture
CIzyme RI (#005-1030)
CIzyme RI is an aseptically filled, lyophilized mixture of 60% purified class I (C1) and 40% purified class II (C2) collagenase from Clostridium histolyticum pre-mixed with neutral protease from Bacillus polymyxa.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 375k CDA U / 75k NP U | Rodent Islet |
CIzyme AS (#005-1090)
GMP Grade CIzyme AS is an aseptically filled, lyophilized mixture of 60% purified class I (C1) and 40% purified class II (C2) collagenase from Clostridium histolyticum pre-mixed with neutral protease from Bacillus polymyxa.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 35 Wünsch U / 280k NP U | Human Adipose |
Purified-Defined Collagenase & BP Protease Mixture
PD Collagenase 100 (#011-3010)
VitaCyte’s purified defined (PD) collagenase products set a new standard of consistency and enables assessment of a broader range of collagenase:protease ratios for cell isolation than found with current collagenase products. Each PD product contains a fixed amount of protease activity and increasing amounts of collagenase activity, simplifying collagenase selection and minimizing the need for lot qualification.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 1 g | Pig Hepatocytes, Rodent Hepatocytes, Stem Cells |
PD Collagenase 800 (#011-3020)
VitaCyte’s purified defined (PD) collagenase products set a new standard of consistency and enables assessment of a broader range of collagenase:protease ratios for cell isolation than found with current collagenase products. Each PD product contains a fixed amount of protease activity and increasing amounts of collagenase activity, simplifying collagenase selection and minimizing the need for lot qualification.
| Package Size | GMP grade | Animal Origin Free | Application |
|---|---|---|---|
| 1 g | Porcine Kidney Epithelial Cells, Rat Cardiomyocytes, Rat Fibroblasts, Rodent Dendritic Cells |
Documentation / FAQ
Download Pack insert, Specification, SDS, CoO and CoA from the following website.
Find the answers to our frequently asked questions below.
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Purified-Defined Collagenase
Purified Neutral Protease
Application Specific Purified-Defined Collagenase Mixture
Purified-Defined Collagenase & BP Protease Mixture
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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The prices are list prices in Japan.Please contact your local distributor for your retail price in your region.