ITEA Fungi Allergen Crude Extracts
Hay fever and house dust allergies are well known, but fungi (mold) are also an important allergen. Mold spores and their metabolic products floating in the air can cause allergic symptoms such as asthma and allergic rhinitis when inhaled.
Alternaria: Has been reported to be associated with the onset and exacerbation of asthma.
Aspergillus: Known as a cause of allergic bronchopulmonary aspergillosis and is also a risk factor for chronic respiratory diseases.
ITEA Inc. provides extracts derived from these fungi, available in two product lines: lyophilized products (containing preservatives) and frozen solutions (0.2 µm filtered, no preservatives).
These products can be used for various applications, including ELISA-based evaluations, cell stimulation tests, and allergy research using model mice.
They manufacture its products with meticulous care under the supervision of experts, leveraging extensive experience and highly precise measurement techniques. Additionally, They offer custom-made products tailored to customer needs, including adjustments to volume, the necessity of lyophilization, and the omission of protectants of preservatives.
Lyophilized Crude extracts
These are lyophilized crude extract made from mold powder containing both mycelium and spores. Reconstitute the lyophilizate in 1mL of distilled water to make a phosphate buffered saline (PBS) solution (pH7.4).
【4-AS-001（Previous code：10118）】Fungi Extract(Aspergillus fumigatus)
- Antigen：
- Aspergillus fumigatus
- Raw material：
- Powder of mold, Aspergillus fumigatus
- Specification：
- No concentration specifications. Only total protein content is measured (varies by lot). Lyophilized
- Composition：
- Extracts from Fungi Extract (Aspergillus fumigatus) (filtered with membranfilter, pore size 0.45 µm), Phosphate-buffered Saline, Contains preservatives
- Storage conditions：
- Store at 2-8 ℃
References
- ElRamlawy, K., Fujimura, T., Baba, T., Kim, J., Kawamoto, C., Isobe, T., Abe, T., Hodge-Hanson, K., Downs, D., Refaat, I., Al-Azhary, D., Aki, T., Asaoku, Y., Hayashi, T., Katsutani, T., Tsuboi, S., Ono, K. and Kawamoto, S.: J. Biol. Chem. 291, 21607(2016). doi: 10.1074/jbc.M116.728006
- Nishikawa, K., Fujimura, T., Ota, Y., Abe, T., ElRamlawy, K., Nakano, M., Takado, T., Uenishi, A., Kawazoe, H., Sekoguchi, Y., Tanaka, A., Ono, K. and Kawamoto, S.: World Allergy Organ. J. 9, 27 (2016). doi: 10.1186/s40413-016-0118-z
【4-AT-001（Previous code：10117）】Fungi Extract (Alternaria alternata)
- Antigen：
- Alternaria alternata
- Raw material：
- Powder of mold, Alternaria Alternata
- Specification：
- No concentration specifications. Only total protein content is measured (varies by lot). Lyophilized
- Composition：
- Extracts from Fungi Extract (Alternaria Alternata) (filtered with membranfilter, pore size 0.45 µm), Phosphate-buffered Saline, Contains preservatives
- Storage conditions：
- Store at 2-8 ℃
References
- Okunishi, K., Wang, H., Suzukawa, M., Ishizaki, R., Kobayashi, E., Kihara, M., Abe, T., Miyazaki, J., Horie, M., Saito, A., Saito, H., Nakae, S., and Izumi, T.: J. Clin. Invest. 130, 3919 (2020). doi:10.1172/JCI127839.
- Morikawa, T., Fukuoka, A., Matsushita, K., Yasuda, K., Iwasaki, N., Akasaki, S., Fujieda, S. and Yoshimoto, T.: Int. Immunol. 29, 221 (2017). doi:10.1093/intimm/dxx030
- ElRamlawy, K., Fujimura, T., Baba, T., Kim, J., Kawamoto, C., Isobe, T., Abe, T., Hodge-Hanson, K., Downs, D., Refaat, I., Al-Azhary, D., Aki, T., Asaoku, Y., Hayashi, T., Katsutani, T., Tsuboi, S., Ono, K. and Kawamoto, S.: J. Biol. Chem. 291, 21607(2016). doi: 10.1074/jbc.M116.728006
- Nomura, A., Kobayashi, T., Seo, W. Ohno-Oishi, M., Kakugawa, K., Muroi, S., Yoshida, H., Endo, T., Moro, K., Taniuchi, I.: life science alliance. 6(8):e202301897, (2023) doi: 10.26508/lsa.202301897.
Frozen solution (0.2 µm filter sterilized, no preservatives)
These are crude extracts of a frozen solution extracted from mold powder (containing both mycelium), which are sterilized through a 0.2 µm filter and contain no protectants or preservatives.
【4-AS-005】Fungi Extract(Aspergillus fumigatus)(0.2 µm filtered, no preservatives)
- Antigen：
- Aspergillus fumigatus
- Raw material：
- Powder of Aspergillus fumigatus
- Specification：
- No concentration specification. Only total protein content is measured (vary by lot). Frozen solution.
- Composition：
- Aspergillus fumigatus crude extract (sterilized with 0.2 µm filter), PBS
- Storage condition：
- Store at -20 ℃ or less (-80 ℃ recommended)
【4-AT-005】Fungi Extract(Alternaria Alternata)（0.2 µm filtered, no preservatives）
- Antigen：
- Alternaria alternata
- Raw material：
- Powder of Alternaria alternata
- Specification：
- No concentration specification. Only total protein content is measured (vary by lot). Frozen solution.
- Composition：
- Alternaria alternata crude extract (sterilized by 0.2 µm filter), PBS
- Storage condition：
- Store at -20 ℃ or less (-80 ℃ recommended)
Information on made-to-order products
If you would like specifications other than those listed in the catalog (such as changes to allergen types, allergen concentrations, volume, whether lyophization is needed, or whether protectans and preservatives is needed), please feel free to contact us.
Desired product information
Unless otherwise specified, ITEA Inc. will extract with PBS and measure the total protein concentration using the Lowry method with BSA as an indicator. The results will be recorded in the analysis certificate.
- Type of extract (pollen / dust mites / insects / mold / others)
- Scientific name (if specified)
- Specification of total protein concentration (e.g., 〇〇 mg/mL)
- Total amount (e.g., total protein mass)
- Amount per unit (if specified) (e.g., 〇〇 mL per unit, 〇〇 mg per unit)
- Solvent specification (PBS / others)
- SDS-PAGE data required / not required
Intended use (as much detail as possible)
ELISA / Cell experiments / Animal experiments / Other (specify)
Other requests
Special notes / Requests for other specification changes:
Q&A
- Where can I obtain an SDS?
- You can download the Safety Data Sheet (SDS) from the link below.
https://www.itea.jp/en/document-download/
- Why is filtration used in the extraction process?
- The purpose of filtration varies depending on the pore size of the filter used. 0.45 µm filter is used to remove impurities, while a 0.2 µm filter is used to filter out microorganisms and other small particles.
- Do the preservatives in the extract affect the ELISA measurement system?
- We add Proclin® 950 as a preservative, but it does not affect the enzymes typically used in ELISA.
- Are the extracts (freeze-dried products) soluble in purified water?
- Yes, they are completely soluble. When dissolved, they become phosphate-buffered saline (PBS, pH 7.4).However, some cloudiness may occur.
- For products that do not specify allergen concentrations or total protein concentrations, can you tell me those concentrations?
- Please contact us as the availability of this service varies depending on the product. In addition, we will charge a separate fee for measurement and delivery.
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Lyophilized extracts
Frozen Solutions (0.2 µm filtered, no preservatives)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.
The prices are list prices in Japan.Please contact your local distributor for your retail price in your region.