Anti HuC/D Antibodies

Hu proteins are RNA-binding proteins, and HuD was identified as the target antigen of autoantibodies present in the serum of patients who developed Paraneoplastic encephalomyelitis¹⁾. In vertebrates, four types -HuR, HuB, HuC, and HuD- have been identified, all of which contain three RNA-binding domains. Their structures are highly conserved, and the Drosophila ELAV (Embryonic Lethal Abnormal Vision) protein is a well-known homolog of the Hu proteins.

Hu proteins are thought to bind primarily to AU-rich elements in mRNA, thereby regulating mRNA stability and translational efficiency. Potential targets of Hu proteins include genes involved in cell proliferation, such as p21 and c-fos, as well as genes associated with neurite outgrowth, such as GAP-43 and Tau²⁾.

Among the Hu proteins, HuB, HuC, and HuD are specifically expressed in neurons and contribute to neuronal differentiation and maintenance by regulating mRNA expression. In immunohistochemistry, anti-HuC/D antibodies are commonly used as neuron-specific markers.

The “Anti HuC/D, Guinea Pig” is a guinea pig polyclonal antibody, raised against HuC/D. It can be used to perform multiplex immunohistochemistry.

Antibody Information

Clonality	Polyclonal
Antigen	Synthetic peptide (internal region of HuC/D)
Host	Guinea pig
Formulation	Antiserum diluted in PBS
Conjugate	Unconjugated
Cross-reactivity	Mouse, Rat
Application	Immunohistochemistry (Frozen Section) 1:1,500

Application Data

Immunohistochemistry

Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,500

Data by courtesy of
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology

Species: Mouse
Site: Cerebral cortex (Layer V)
Sample: Frozen section
Antibody concentration: 1:1,500

Data by courtesy of
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology

Species: Mouse
Site: Hippocampus (CA3)
Sample: Frozen section
Antibody concentration: 1:1,500

Data by courtesy of
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology

1. Szabo, A. et al.: Cell, 67(2), 325(1991).
   HuD, a paraneoplastic encephalomyelitis antigen, contains RNA-binding domains and is homologous to Elav and Sex-lethal
2. Akamatsu, W. et al.: Proc. Nat. Acad. Sci. USA, 102(12), 4625(2005).
   The RNA-binding protein HuD regulates neuronal cell identity and maturation