CultureSure™ Small Molecules
CultureSure™ small molecules are reportedly involved in the processes of ES and iPS cell differentiation and maintaining their capacity to remain undifferentiated. The products are safe to be used in cell culture as their level of cytotoxicity has beenchecked and they have undergone mycoplasma and endotoxin tests. 1 mL preparation of Y-27632 solution is now available for use.
Product Line Up
A419259 Trihydrochloride
A-83-01
ALK5 InhibitorⅡ
CHIR99021
CKI-7 Dihydrochloride
DAPT
Dorsomorphin
Forskolin
IWP-2 & IWR-1-endo
PD0325901
Purmorphamine
KY03-Ⅰ
SB431542
3,3',5-Triiodo-L-thyronine Sodium Salt (T3)
Y-27632
A419259 Trihydrochloride
C29H34N6・3HCl=592.00
What is A419259?
SA419259 is an inhibitor of Src family kinases. Src is involved in the production of tyrosine kinases that specifically phosphorylate tyrosine residues of proteins. Src family kinases are considered to play important roles in cell development, differentiation and proliferation signaling pathways.
※ Tested for endotoxin, tested negative for mycoplasma
Product Description
- Appearance: white to pale yellowish red, crystalline powder to powder
- Solubility: soluble in water, DMSO, ethanol, acetonitrile + water (1:1 ratio)
- Tested negative for mycoplasma
- Endotoxin: less than 0.1 EU/mg
- Storage: room temperature (under inert gas)
A-83-01
C25H19N5S=421.52
What is A-83-01?
A-83-01 is a selective inhibitor of ALK4, ALK5, and ALK7. It blocks phosphorylation of Smad2/3 and inhibits TGF-β-induced epithelial-to-mesenchymal transition. This product has little to no effect on bone morphogenetic protein type I receptors, p38 MAP kinase, and extracellular signal-regulated kinases. A-83-01 is known to help rat iPS cellscan be long-term cultured in a state maintaining the undifferentiated state.
※ Tested for endotoxin, tested negative for mycoplasma
※ Checked for cell cytotoxicity (using human iPS cell line 201B7)
Product Description
- Assay (HPLC): min. 98.0%
- Appearance: white to yellow, crystalline
- Ppowder to powder or mass
- Solubility: soluble in DMSO
- Tested negative for mycoplasma
- Endotoxin: less than 0.01 EU/mg
- Storage: -20°C in the dark (under inert gas)
References
Li, W., et al. : Cell Stem Cell, 4, 16 (2009).
Katsuda ,T., et al. : Cell Stem Cell, 20, 41 (2017).
Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).
ALK5 InhibitorⅡ
What is ALK5 Inhibitor Ⅱ?
ALK5 Inhibitor Ⅱ is a TGF-βR I kinase/ALK5 inhibitor. It can be used as a substitute for Sox2 or c-Myc when generating mouse iPS cells.
※ Tested for endotoxin, tested negative for mycoplasma
Product Description
- Assay (HPLC): min. 98.0%
- Appearance: white to yellowish brown, crystals to powder
- Solubility: soluble in DMSO
- Tested negative for mycoplasma
- Endotoxin: less than 0.05 EU/mg
- Storage: 2-10℃ in the dark (under inert gas)
References
Maherali, N., et al. : Curr. Biol., 19, 1718 (2009).
CHIR99021
C22H18Cl2N8=465.34
What is CHIR99021?
CHIR99021 is a highly selective inhibitor of GSK-3β. The product does not exhibit cross-reactivity against CDKs.
CHIR99021 has been used to maintain effectively undifferentiated ES cells in combination with PD0325901.
※ Tested for endotoxin, tested negative for mycoplasma
※ Checked for cell cytotoxicity (using human iPS cell line 201B7)
Product Description (Powder Form)
- Assay (HPLC): min. 97.0%
- Appearance: white to pale brown, crystalline powder to powder
- Solubility: soluble in DMSO and methanol
- Tested negative for mycoplasma
- Endotoxin: less than 0.05 EU/mg
- Storage: -20°C in the dark
Product Description (Liquid Form)
- Appearance: liquid
- Concentration: dissolved in DMSO, 10 mmol/L
- Sterilized with 0.1 μm filter
- Tested for sterility
- Tested negative for mycoplasma
- Tested for endotoxin: less than 2 EU/mL (for the first lot)
- Storage: -20°C in the dark
- No animal-derived components were used in the raw materials and in the manufacturing processes
Reference
Ying, QL., et al .: Nature, 453, 519 (2008).
CKI-7 Dihydrochloride
C11H12ClN3O2S・2HCl=358.67
What is CKI-7 Dihydrochloride?
CKI-7 dihydrochloride is one of the isoquinoline sulfonamide, and is an ATP-competitive inhibitor that selectively inhibit casein kinase 1 (CK1). As a serine/ threonine kinase, CK1 regulates various processes such as nuclear export, cell cycle, cell division, and apoptosis. CKI-7 dihydrochloride is known for induce retinal progenitor cells from human ES and iPS cells in combination with SB431542 and Y-27632 under serum-free and feeder-free conditions.
※ Tested for endotoxin, tested negative for mycoplasma
※ Checked for cell cytotoxicity (using human iPS cell line 201B7)
Product Description (Powder Form)
- Assay (HPLC): min. 98.0%
- Appearance: white to pale brown, crystalline powder to powder
- Solubility: soluble in water
- Tested negative for mycoplasma
- Endotoxin: less than 0.05 EU/mg
- Storage: -20°C in the dark (under inert gas)
Product Description (Liquid Form)
- Appearance: liquid
- Concentration: dissolved in water, 3 mmol/L
- Sterilized with 0.1 μm filter
- Tested for sterility
- Tested negative for mycoplasma
- Tested for endotoxin: less than 1 EU/mL (for the first lot)
- Storage: -20°C in the dark
- No animal-derived components were used in the raw materials and in the manufacturing processes
Reference
Takahashi, M. et al.: J. Cell Sci., 122, 3169 (2009).
DAPT
What is DAPT?
DAPT is a γ-secretase inhibitor that inhibits Notch signaling. It induces neural differentiation from ES cell embryoid bodies and preferentially induces cone photoreceptor subtypes in retinal cell differentiation. It is also used as a component of medium when differentiation of brain organoid.
※ Tested for endotoxin, tested negative for mycoplasma
Product Description
- Assay (HPLC): min. 99.0%
- Appearance: white to slightly yellowish brown, crystalline powder to powder or mass
- Solubility: soluble in DMSO
- Tested negative for mycoplasma
- Endotoxin: less than 0.25 EU/mg
- Storage: -20℃
References
Crawford, TQ., et al. : Dev. Dyn., 236, 886 (2007).
Nelson, BR., et al. : Dev. Biol., 304, 479 (2007).
Zerti, D., et al. : Stem Cells, 38, 45 (2020).
Dorsomorphin
What is Dorsomorphin?
This is a pyrazolopyrimidine compound with cellular permeability. It acts as a potent, selective, reversible, and ATP-competitive inhibitor of AMPK.
It induces neural differentiation from human iPS cells, and cardiomyocytes from mouse ES cells.
It has been reported that human brainstem organoids can be derived from human iPS/ES cells when used in combination this with several other small molecule compounds.
※ Tested for endotoxin, tested negative for mycoplasma
Product Description
- Assay (HPLC): min. 98.0%
- Appearance: white to yellow, crystals to powder
- Solubility: soluble in DMSO
- Tested negative for mycoplasma
- Endotoxin: less than 0.25 EU/mg
- Storage: -20℃ (under inert gas)
References
Chen, Y., et al. : Methods Mol. Biol., 1919, 59 (2019).
Hao, J., et al. : PLOS ONE, 3, e2904 (2008).
Nobuyuki, E., et al. : Front Neurosci., 14, 538 (2020).
Forskolin
What is Forskolin?
Forskolin is an activator of adenylate cyclase. It induces differentiation of mesenchymal stem cells into neurons when used in conjunction with bFGF. It is also used in culture liver organoids.
※ Tested for endotoxin, tested negative for mycoplasma
Product Description
- Assay (HPLC): min. 98.0%
- Appearance: white to slightly yellowish brown, crystalline powder to powder or mass
- Solubility: soluble in DMSO
- Tested negative for mycoplasma
- Endotoxin: less than 0.25 EU/mg
- Storage: 2-10℃
References
Jang, S., et al. : BMC Cell Biol., 11, 25 (2010).
IWP-2 & IWR-1-endo
CultureSure® IWP-2
C22H18N4O2S3=466.60
What is IWP-2?
IWP-2 inactivates porcupine (Porcn), a membrane-bound o-acyltransferase (MBOAT), and suppresses palmitoylation of Wnt proteins. As a result, it blocks phosphorylation of Wnt-dependent Lrp-6 receptor and Dvl2, and accumulation ofβ-catenin. It is known that it promotes differentiation of human iPS cells into cardiomyocytes.
※ Tested negative for mycoplasma
※ Checked for cell cytotoxicity (using human iPS cell line 201B7)
Product Description
- Assay (HPLC): min. 98.0%
- Appearance: white to pale brown, crystals to powder
- Solubility: soluble in DMSO
- Tested negative for mycoplasma
- Storage: 2-10 °C in the dark (under inert gas)
Reference
Minami, I. et al.: Cell Rep., 2, 1448 (2012).
CultureSure® IWR-1-endo
C25H19N3O3=409.44
What is IWR-1-endo?
IWR-1-endo is an inhibitor of Wnt signaling. IWR-1 stabilizes the complex composed of Axin2, Apc, Ck1, and Gsk3β that degrades β-catenin. As a result, it promotes degradation of β-catenin.
※ Tested for endotoxin, tested negative for mycoplasma
Product Description
- Assay (HPLC): min. 98.0%
- Appearance: white to pale yellow, crystalline powder to powder
- Solubility: soluble in DMSO
- Tested negative for mycoplasma
- Storage: -20 °C in the dark (under inert gas)
PD0325901
What is PD0325901?
PD0325901 is a MAPK inhibitor with potent antitumor activity.
It induces phosphorylation of MAPK, inhibition of its activation and inhibition of tumor cell proliferation. PD0325901 inhibits MAPK activity without adverse reactions.
Addition of this product and CHIR99021 to a culture medium results in efficient culture of mouse ES cells.
It has been reported that use of this product with SB431542 and thiazovivin not only improves reprogramming efficiency 200 folds or more but also accelerates reprogramming speed.
※ Tested for endotoxin, tested negative for mycoplasma
Product Description
- Assay (HPLC): min. 99.0%
- Appearance: white to pale brown, crystals to powder
- Solubility: soluble in DMSO
- Tested negative for mycoplasma
- Endotoxin: less than 0.25 EU/mg
- Storage: -20℃ (under inert gas)
References
Lin, T., et al. : Nat. Methods, 6, 805 (2009).
Ying, QL., et al. : Nature, 453, 519 (2008).
Koehler, KR., et al. : Nat. Protoc., 9, 1229 (2014).
Purmorphamine
What is Purmorphamine?
Purmorphamine is an agonist of hedgehog signaling. Induces differentiation of mesenchymal progenitor cells and preosteoblast cells into osteoblasts.
※ Tested for endotoxin, tested negative for mycoplasma
Product Description
- Assay (HPLC): min. 98.0%
- Appearance: white to pale brown or bluish purple, crystalline powder to powder or mass
- Solubility: soluble in DMSO
- Tested negative for mycoplasma
- Endotoxin: less than 0.25 EU/mg
- Storage: -20℃
References
Wu, X., et al. : Chem. Biol., 11, 1229 (2004).
KY03-Ⅰ
C19H19IN2O3S=482.34
What is KY03-I?
KY03-I is an inhibitor of Wnt. It is known that KY02111 induces differentiation of human ES and iPS cells into cardiomyocytes. Compared with KY02111, KY03-I induces differentiation of ES/ iPS cells into cardiomyocytes in a more efficient manner and at a lower concentration.
※ Tested for endotoxin, tested negative for mycoplasma
Product Description
- Assay (HPLC): min. 98.0%
- Appearance: white to pale gray, crystalline powder to powder
- Solubility: soluble in DMSO, ethanol, acetone, and methanol
- Tested negative for mycoplasma
- Endotoxin: less than 0.01 EU/mg
- Storage: -20 °C in the dark (under inert gas)
Reference
Minami, I. et al.: Cell Rep., 2, 1448 (2012).
SB431542
C22H16N4O3=384.39
What is SB431542?
SB431542 is a selective inhibitor of TGF-β superfamily type 1 activin receptor-like kinase (ALK) and inhibits ALK4, 5, and 7. It is known that it stimulates proliferation, differentiation and sheet formation of ES cells-derived endothelial cells. It is also known that the use of SB431542 with thiazovivin and PD0325901 improves reprogramming efficiency to more than 200 times and accelerates reprogramming speed.
※ Tested for endotoxin, tested negative for mycoplasma
※ Checked for cell cytotoxicity (using human iPS cell line 201B7)
Product Description (Powder Form)
- Assay (HPLC): min. 98.0%
- Appearance: white to slightly yellowish brown, crystalline powder to powder
- Solubility: soluble in DMSO, acetonitrile, acetone, and ethanol
- Tested negative for mycoplasma
- Endotoxin: less than 0.05 EU/mg
- Storage: -20 °C in the dark
Product Description (Liquid Form)
- Appearance: liquid
- Concentration: dissolved in DMSO, 5 mmol/L
- Sterilized with 0.1 μm filter
- Tested for sterility
- Tested negative for mycoplasma
- Tested for endotoxin: less than 2 EU/mL (for the first lot)
- Storage: -20°C in the dark
- No animal-derived components were used in the raw materials and in the manufacturing processes
References
Ogawa, K., et al. : J. Cell. Sci.,120, 55 (2007).
Lin T., et al. : Nat. Methods, 6, 805 (2009).
Fujimori, K., et al. : Stem Cell Reports., 9, 1 (2017).
Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).
3,3',5-Triiodo-L-thyronine Sodium Salt (T3)
What is 3,3',5-Triiodo-L-thyronine Sodium Salt (T3)?
It is one of the thyroid hormones. It signals by binding to nuclear receptors and regulating the expression of a wide range of gene products.
It promotes differentiation of oligodendrocyte progenitor cells or maturation of human iPS cell-derived cardiomyocytes. Thyroid hormone signaling has also been reported to act on cone subtype determination in retinal cell differentiation.
※ Tested for endotoxin, tested negative for mycoplasma
Product Description
- Assay (HPLC): min. 98.0%
- Appearance: white to pale yellowish brown or reddish brown, crystalline powder to powder
- Solubility: soluble in DMSO
- Tested negative for mycoplasma
- Endotoxin: less than 0.25 EU/mg
- Storage: -20℃ (under inert gas)
References
Marta, CB., et al. : J. Neurosci. Res., 53, 251 (1998).
Yang, X., et al. : J. Mol. Cell. Cardiol., 72, 296 (2014).
Eldred, KC., et al. : Science, 362, eaau6348 (2018).
Y-27632
C14H21N3O・2HCl・H2O=338.27
What is Y-27632?
Y-27632 is a selective and potent inhibitor of ROCK. It induces various activities such as contraction of vascular smooth muscle cells via ROCK signaling pathways. Furthermore, it suppresses the cell death of of ES and iPS cells at cell dispersion, and that it improves cell survival after cryopreservation. [ROCK：Rho-associated coiled-coil forming kinase / Rho-linked kinase/ serine/threonine protein kinase]
※ Tested for endotoxin, tested negative for mycoplasma
※ Checked for cell cytotoxicity (using human iPS cell line 201B7)
Product Description (Powder Form)
- Assay (HPLC): min. 98.0%
- Appearance: white to pale yellow, crystalline powder to powder
- Solubility: soluble in water and ethanol
- Specific rotation [α]D20(c=0.5,CH3OH) ：+6.3°(for the first lot)
- Tested negative for mycoplasma
- Endotoxin: less than 0.25 EU/mg
- Storage: -20 °C in the dark (under inert gas)
Product Description (Liquid Form)
- Appearance: liquid
- Concentration: dissolved in water, 10 mmol/L
- Sterilized with 0.1 μm filter
- Tested for sterility
- Tested negative for mycoplasma
- Tested for endotoxin: less than 0.78 EU/mL (for the first lot)
- Storage: -20°C in the dark
- No animal-derived components were used in the raw materials and in the manufacturing processes
Our Y-27632 products lineup (including GMP products)
References
Uehata, M., et al. : Nature, 389, 990 (1997).
Sakamoto, K., et al. : J. Pharmacol. Sci., 92, 56 (2003).
Nishimaru, K., et al. : J. Pharmacol. Sci., 92, 424 (2003).
Watanabe, K., et al. : Nat. Biotechnol., 25, 681 (2007).
Martin-Ibanez, R., et al. : Hum. Reprod., 23, 2744 (2008).
Claassen, DA., et al. : Mol. Reprod. Dev., 76, 722 (2009).
Kawamata, M., et al. : Proc. Natl. Acad. Sci. USA., 107, 14223 (2010).
Ito, H., et al. : Liver Int., 32, 592 (2012).
Katsuda,T., et al. : Cell Stem Cell, 20, 41 (2017).
Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).
Ogawa, K., et al. : Sci. Rep., 8, 3615 (2018).
Ayabe, H., et al. : Stem Cell Reports., 11, 306 (2018).
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