Adiponectin ELISA Kits
Adiponectin is a cytokine secreted by adipocytes and plays a role in regulating lipid metabolism and insulin sensitivity and is known for its antidiabetic, anti-atherosclerotic, and anti-inflammatory effects. In the bloodstream, adiponectin exists in multimeric forms: trimers (low molecular weight, LMW), hexamers (medium molecular weight, MMW), and higher-order complexes of 12 to 18 monomers (high molecular weight, HMW). Circulating HMW adiponectin levels, rather than total adiponectin, are considered a more accurate indicator of physiological parameters such as BMI, sex, weight loss, glucose tolerance, hepatic insulin sensitivity, and the presence of metabolic syndrome or type 2 diabetes.
The LBIS™ Mouse/Rat High Molecular Weight Adiponectin ELISA Kit is a sandwich ELISA kit designed for the quantitative determination of HMW adiponectin in mouse and rat samples. It utilizes two specific antibodies to selectively capture and detect HMW adiponectin.
What is Adiponectin?
Adiponectin is a cytokine secreted by adipocytes and classified as an adipocytokine (also referred to as an adipokine). It plays a role in regulating lipid metabolism and insulin sensitivity and is known for its antidiabetic, anti-atherosclerotic, and anti-inflammatory effects. Adiponectin also binds various growth factors and modulates processes such as cell proliferation, angiogenesis, and tissue remodeling by sequestering these factors.
In the bloodstream, adiponectin exists in multimeric forms: trimers (low molecular weight, LMW), hexamers (medium molecular weight, MMW), and higher-order complexes of 12 to 18 monomers (high molecular weight, HMW). Trimers are formed through non-covalent interactions involving the triple-helical structure in the collagenous domain and hydrophobic interactions in the globular C1q domain. Hexamers and larger complexes are generated through the oligomerization of trimers.
Circulating HMW adiponectin levels, rather than total adiponectin, are considered a more accurate indicator of physiological parameters such as BMI, sex, weight loss, glucose tolerance, hepatic insulin sensitivity, and the presence of metabolic syndrome or type 2 diabetes.
LBIS™ Mouse/Rat High Molecular Weight Adiponectin ELISA Kit
The LBIS™ Mouse/Rat High Molecular Weight Adiponectin ELISA Kit is a sandwich ELISA kit designed for the quantitative determination of HMW adiponectin in mouse and rat samples. It utilizes two specific antibodies to selectively capture and detect HMW adiponectin. All reagents are supplied in liquid form and are ready to use.
Kit Performance
| Assay target | High Molecular Weight Adiponectin |
|---|---|
| Calibration curve range | 3.13-200 ng/mL |
| Analysis sample | Mouse Serum/Plasma Rat Serum/Plasma Cell Culture Supernatant |
| Sample volume | 50 μL (25 to 50-fold dilution) |
| Measurement duration | Approx. 4 hours |
| Wavelength | Primary wavelength 450 nm Reference wavelength 620 nm |
Example of Calibration Curve
Cross-reactivity with Similar Proteins
| Animals | Proteins | Cross-reactivity (%) |
|---|---|---|
| Mouse | Adiponectin (HMW) | 100 |
| Adiponectin (Hexamer) | < 5 | |
| Adiponectin (Trimer) | Less than sensitivity | |
| Adiponectin (Monomer) | Less than sensitivity | |
| MCH | Less than sensitivity | |
| TNF-α | Less than sensitivity | |
| IFN-γ | Less than sensitivity | |
| Insulin | Less than sensitivity | |
| Leptin | Less than sensitivity |
| Animals | Proteins | Cross-reactivity (%) |
|---|---|---|
| Rat | Adiponectin (HMW) | 100 |
| Adiponectin (Monomer) | Less than sensitivity | |
| TNF-α | Less than sensitivity | |
| IFN-γ | Less than sensitivity | |
| Insulin | Less than sensitivity | |
| Leptin | Less than sensitivity |
Example: Measurement of adiponectin in mouse/rat serum
| Animals | Strains | Age (week) |
Sex | Number of sample | Measured value (ng/mL) |
SD |
|---|---|---|---|---|---|---|
| Mouse | BALB/c | 6 | Male | 10 | 2,369 | 743 |
| ICR | 6 | Male | 10 | 2,119 | 802 | |
| BKS.Cg-+Leprdb/+Leprdb/ | 12 | Male | 10 | 1,802 | 875 | |
| KKAy/Ta | 12 | Male | 16 | 2,141 | 937 | |
| B6.V-Kepob | 12 | Male | 10 | 1,229 | 437 | |
| Rat | CD (SD) | 8 | Male | 9 | 3,220 | 670 |
| GK | 12 | Male | 10 | 3,512 | 304 | |
| ZUC-Leprfa | 8 | Male | 10 | 1,991 | 1,120 |
Sample
Serum
Blood sampling conditions
Fasting : None
Anesthesia : Isoflurane
Site : Heart (Abdominal aorta in KKAy/Ta mice)
Rearing conditions
Only KKAy/Ta mice were single-housed.
Example: Measurement of HMW adiponectin in male and female mice
Mean ± SE, **: P＜0.01Sample
Serum
Blood sampling conditions
Fasting : 24 hours
Anesthesia : Isoflurane
Site : Heart
Food
Normal Food (CRF-1)
Rearing conditions
Group-Housing
Changes in HMW adiponectin levels following pioglitazone administration
| Animal | Mouse (KKAy/Ta, Male, 7-weeks old) |
| Food | CE-2 supplemented with 20% soybean oil |
| Test | 10 mg/10 mL/kg Pioglitazone (n=6) |
| Control | 10 mL/kg Saline (n=6) |
Drug administration
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Adiponectin (High Molecular Weight) ELISA Kit (Mouse/Rat)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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