Wakopak^® Handy ODS, Wakopak^® eco-ODS

Features

• Secure sufficient performance for advanced analysis
  4.6 mm x 150 mm: N= more than 12,000
  4.6 mm x 250 mm: N= more than 20,000
• High-purity silica gel is used and is optimal for the separation of metal coordination compounds
• Alredy endcapped, it is optimal for basic compound separation
• Compatible with a wide range of analyses to provide typical retention capabilities by monomeric ODS
• Improves column life by low column pressure
• Low cost similar to that of guard column
• Rapid flow rate
  [Physical properties] mean particle size: 6 μm, mean pore size: 12 nm, %C (carbon content): 16%

　

Application

Essential performance data for Wakopak^® Handy ODS

Essential performance evaluation data of column

• 

• Column Eluent Flow rate Detection

  ：Wakopak® Handy ODS (4.6 mm × 250 mm) ：CH3CN/H2O=60/40 (v/v) ：1.0mL/min. at R.T. ：UV 254 nm 　N=23,000 　K=3.89 　α=2.07 　S=1.07

  *Theoretical plate number (N) and symmetry factor (S) are calculated from Naphthalene.

　

Endcapping data

• 

• Column Eluent Flow rate Detection

  ：Wakopak® Handy ODS (4.6 mm × 250 mm) ：CH3CN/10 mM K2HPO4 (pH7.0)=50/50 (v/v) ：1.0 mL/min. at R.T. ：UV 254 nm

　

Analysis of polyphenols by Wakopak^® Handy ODS

Features

• Simplified packaging materials
• Broadly compatible with analysis of a variety of compounds, from acidic to basic
• The column pressure is low, and it is easy to use this column with methanol as the solvent.
• Reasonable pricing

* Base material of the packing agent: total porous high-purity silica gel with an average particle size of 5 μm
* Chemical modification: ODS (monomeric type, high-endcapping treatment)

　

Application

Analysis of compounds by Wakopak^® eco-ODS

Basic compounds

• 

• Eluent Flow rate Detection Injection volume

  ：CH3CN/10 mM KH2PO4 (pH 7.0)＝30/70 (v/v) ：1.0 mL/min. at 35℃ (Press: 48 bar) ：UV 254 nm ：10 μL

• 

• Eluent Flow rate Detection Injection volume

  ：CH3CN/H2O＝30/70 (v/v) ：1.0 mL/min. at 30℃ (Press: 68 bar) ：UV 254 nm ：1 μL

• 

• Eluent Flow rate Detection Injection volume

  ：20 mM Phosphate buffer (pH7)/CH3OH＝70/30 (v/v) ：1.0 mL/min. at 30℃（Press: 76 bar） ：UV 254 nm ：1 μL

　

Acidic compounds

• 

• Eluent Flow rate Detection Injection volume

  ：20 mM NaH2PO4 (pH2.5) ：1.0 mL/min. at 35℃ (Press: 44 bar) ：UV 210 nm ：2 μL

　

Coordinating compounds

• 

• Eluent Flow rate Detection Injection volume

  ：CH3OH/20 mM K2HPO4 (pH7.6)＝60/40 (v/v) ：1.0 mL/min. at 30℃ (Press: 77 bar) ：UV 254 nm ：5 μL

　

Food preservatives

• 

• Eluent Flow rate Detection Injection volume

  ：CH3OH/CH3CN/5 mM Citric buffer (pH4)＝10/20/70 (v/v/v) ：1.0 mL/min. at 40℃ (Press: 51 bar) ：UV 230 nm ：5 μL

　

Active ingredients of crude drugs

• 

• 	Compliant with the Japanese Pharmacopoeia 17th Edition
  Eluent	KH2PO4,Triethylamine (pH3.5)*1/CH3CN=90/10 (v/v)
  Flow rate	1.0 mL/min. at 25℃ (Press: 58 bar)
  Detection	UV 210 nm
  Injection volume	6 μL

  *1: 6.8 g of KH₂PO₄ was dissolved in 900 mL of water, 10 mL of (C₂H₅)₃N was added, the pH was adjusted to 3.5 with H₃PO₄, and water was added to adjust the volume to 1,000 mL.