C18 column
Wakopak® Wakosil®-Ⅱ 5C18, 5C18-100
Wakopak® Wakosil®- II 5C18 and 5C18-100 use high-purity silica gel with different pore sizes as the raw material.
The carbon content of Wakosil®-II 5C18-100 is as high as 22%, showing high retention.
Applications
Analysis of Aniline Derivatives
| Column Eluent Flow Rate Detection |
：Wakopak® Wakosil®-Ⅱ 5C18 (4.6 mm × 150 mm) ：CH3OH/20 mM KH2PO4-H3PO4 buffer (pH2.7) = 5/95 (v/v) ：1.0 mL/min. at 30℃ ：UV 254 nm |
Analysis of Vitamin B1 Derivatives
| Column Eluent Flow Rate Detection |
：Wakopak® Wakosil®-Ⅱ 5C18 (4.6 mm × 150 mm) ：7.5 mM NaH2PO4 buffer ：1.0 mL/min. at 40℃ ：UV 270 nm |
Analysis of the Active Ingredient in Scutellaria Root
| Column Eluent Flow Rate Detection |
：Wakopak® Wakosil®-Ⅱ 5C18-100 (4.6 mm × 150 mm) ：A）0.1 M H3PO4 B）CH3CN/0.1 M H3PO4 (v/v) = 50/50 (v/v) 0–20 min. B conc. 40–100％ 20–30 min. B conc. 100％ ：1.0 mL/min. at 50℃ ：UV 280 nm 0.16 AUFS |
Analysis of Hinokitiol in Hair Tonics
| Column Eluent Flow Rate Detection |
：Wakopak® Wakosil®-Ⅱ 5C18-100 (4.6 mm × 150 mm) ：CH3CN/H2O containing 0.05% EDTA・2Na = 60/40 (v/v) ：1.0 mL/min. at RT ：UV 254 nm 0.32 AUFS |
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Wakopak® Wakosil®-Ⅱ 5C18
ODS column for reverse-phase chromatography.
Wakopak® Wakosil®-Ⅱ 5C18-100
ODS column for reverse-phase chromatography.
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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