Wakopak® Wakosil® 5TMS
Wakopak® Wakosil® 5TMS is a HPLC column using revers-phase packing agent composed of trimethylsilyl groups (TMS) chemically bonded to silica gel. The retention of hydrophobic compounds is less than that of butyl (C4).
Wakopak® Wakosil® 5TMS is effective when you want to quickly elute compound with a stronger retention that other reverse-phase packing agent cannot or when you cannot increase the ratio of organic solvents in the mobile phase. Wakopak® Wakosil® 5TMS is also used for simultaneous analysis of samples with a wide range of polarity.
Applications
Comparison of Retention of Each Packing Agent in Neutral Compounds Analysis
| Column Size Eluent Detection |
：4.6 mm × 150 mm ：CH3CN/H2O = 60/40 (v/v) ：UV 254 nm |
|
Analysis Example of Water-Soluble Vitamins
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Column
Eluent
Flow Rate
Detection：Wakopak® Wakosil® 5TMS (4.6 mm × 150 mm)
：CH3OH/0.15 mM KH2PO4 = 5/95 (v/v)
：1.0 mL/min. at R.T.
：UV 254 nm
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Wakopak® Wakosil® 5TMS
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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