PYROSTAR™ ES-F series (LAL Gel-clot and Turbidimetric Reagent)
Endotoxin-specific LAL reagents (not activated by (1→3)-β-D-glucan), compatible with the BET (USP) compliance tests U.S Food and Drug Administration (FDA) licensed. Intended Use: Limulus amebocyte lysate (LAL) is intended for the detection of gram-negative bacterial endotoxins. PYROSTAR™ ES-F is intended for the qualitative detection of endotoxins by gel-clot or quantitative detection by kinetic turbidimetric methods.
- Endotoxin-specific lysate, avoids false positive results from glucans
- Available in multi-tests vials or single-test vials
- Can be used as either a gel-clot or Kinetic-Turbidimetric Assay (KTA) reagent
- KTA can be performed in tube reader
- Gel-Clot lysate sensitivities range from 0.015 to 0.25 EU/mL
- Available with matched control standard endotoxin (CSE)
- PYROSTAR™ ES-F reagents (Gel-clot sensitivity 0.015 EU/mL) are available with a KTA quantitative range of either 0.001 EU/mL to 10 EU/mL.
The KTA quantitative range is related to the Gel-Clot sensitivity. - Multi test is performed by mixing 0.1 mL of lysate reagent with 0.1 mL of sample, whereas in single test, a 0.2 mL sample directly dissolves lysate reagent.
| Single test: | The lysate reagent for one measurement is dispensed in advance in a test tube. |
|---|---|
| Multi test: | The lysate reagent which dissolve in the endotoxin test water, dispense the necessary amount in reaction tubes. |
PYROSTAR™ ES-F Single Test Vial
| Gel-clot Sensitivity(EU/mL) | KTA Quantitative Range(EU/mL) |
|---|---|
| 0.015 | 0.001 to 10 |
| 0.03 | 0.01 to 10 |
PYROSTAR™ ES-F Multi Test Vial with CSE
| Gel-clot Sensitivity(EU/mL) | KTA Quantitative Range(EU/mL) |
|---|---|
| 0.015 | 0.001 to 10 |
| 0.03 to 0.25 | 0.01 to 10 |
PYROSTAR™ ES-F Multi Test Vial without CSE (Bulk)
| Gel-clot Sensitivity(EU/mL) | KTA Quantitative Range(EU/mL) |
|---|---|
| 0.015 | 0.001 to 10 |
| 0.03 to 0.25 | 0.01 to 10 |
Standard curve information
Regression equation: log(Tg) = -0.289200 log(C) +1.13012
Correlation coefficient: -0.9984-
Endotoxin concentration(EU/mL) Gelation time (min.) 0.001000 103.8
103.8
103.60.01000 49.2
49.0
48.80.1000 25.2
25.2
25.21.000 14.0
14.0
14.2
Recovery rate of spiked endotoxins with the Turbidimetric technique
-
Single test
-
Multi test
*Multi test is performed by mixing 0.1 mL of lysate reagent with 0.1 mL of sample, whereas in single test, a 0.2 mL sample directly dissolves lysate reagent. Therefore, there will be a difference in sample concentration in the reaction solution. In general, single test tend to be more affected by samples.
Wako Blog: Talking of LAL
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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