ITEA Mite Extract for Asthma Mouse Models
ITEA Inc. offers allergen extracts containing house dust mites(HDM) that are associated with asthma.
These products do not contain preservatives, these are suitable for use in the production of asthma model mice.
Features
- No adjuvants added
- Protectants or preservatives free.
- Total protein content 1 mg/vial
- Crude extract (filtered through a 0.2 µm filter)
- 1 mL frozen PBS solution (pH 7.4)
Example of use in the creation of asthma model mice (#4-DF-003 Dust mite (Df) extract (for immunological use))
Experimental examples of #4-DF-003, House Dust Mite Extract For Immunization (Df)
Experiment on the generation of HDM-induced airway inflammation model mice using BALB/c.
Immunization schedule
Mouse : Female BALB/c mice, 6‒8 weeks old
- Mice were administered intranasally with 25 µg as total protein of House Dust Mite Extract For Immunization (Df) (Df extract) on days 1, 8 and 15.
- Mice were challenged intranasally with 5 µg as total protein of Df extract on days 22, 23 and 24.
- 24 hours after the last intranasal dose, bronchoalveolar lavage fluid (BALF), lung histopathology, airway hyperresponsiveness, and pulmonary cytokine production were assessed.
Cell counts in BAL fluids
Pathological findings of the pulmonary bronchi
Results may vary depending on your experimental environment, techniques, and mouse condition.
Experiment on the generation of asthma model mice using C57BL/6.
Immunization schedule
Mouse : Female C57BL/6 mice, 6‒8 weeks old
- House Dust Mite Extract For Immunization (Df) was administered intranasally to the mice at a total protein content of 30 µg once every 2 to 3 days for a total of 7 times.
- 24 hours after the last intranasal dose, BALF, lung histopathology, airway hyperresponsiveness, and IL-13 mRNA expression in the lung were assessed.
Cell counts in BAL fluids
Pathological findings of the pulmonary bronchi
Results may vary depending on your experimental environment, techniques, and mouse condition.
House Dust Mite Crude Extracts for Immunization
House Dust Mite Extract For Immunization (Df)
This is a crude extract (frozen solution) of the house dust mite (scientific name: Dermatophagoides farinae; DF).
Features
- Including 1 mg/vial of the total proteins. (measured by Lowry protein assay with BSA standard)
- Filtered with membranfilter, pore size 0.2 µm for sterilization.
- Not including any adjuvants or preservatives.
References
- Nunokawa H, et al.: Sci. Rep. 11, 13157 (2021). DOI: 10.1038/s41598-021-92561-w
- Ookawara T, et al.: Toxicol. Sci. 179(2), 229 (2021). DOI: 10.1093/toxsci/kfaa168
- Murakami Y, et al.: Sci. Rep. 10, 1 (2020). DOI: 10.1038/s41598-020-75153-y
- Sadamatsu H, et al.: Int. Arch. Allergy Immunol.181(9), 665 (2020) DOI: 10.1159/000508709
- Sadamatsu H, et al.: Inflamm. Res. 69, 139 (2020). DOI: 10.1007/s00011-019-01302-3
- Tajima H, et al.: J. Appl. Toxicol. 40(11), 1545 (2020). DOI: 10.1002/jat.4017
- Ishii T, et al.: Scand. J. Immunol. 87 (2018). DOI: 10.1111/sji.12641
- Tashiro H et al.: Inflammation, 40(3), 1072 (2017). DOI: 10.1007/s10753-017-0550-4
- Tashiro H et al.: I. PLoS One. 11(6), e0157571 (2016). DOI: 10.1371/journal.pone.0157571
- Kawano H, et al.: Int. Immunol. 28(10), 489 (2016). DOI: 10.1093/intimm/dxw012
- Kato G, et al.: BMC Immunol. 15(1), 39 (2014). DOI: 10.1186/s12865-014-0039-y
House Dust Mite Extract For Immunization (Dp)
This is a crude extract (frozen solution) of the house dust mite (scientific name: Dermatophagoides pteronyssinus; DP).
Features
- Including 1 mg/vial of the total proteins. (measured by Lowry protein assay with BSA standard)
- Filtered with membranfilter, pore size 0.2 µm for sterilization.
- Not including any adjuvants or preservatives.
References
- Kawasaki T, et al.: EMBO J, 36(12), 1707(2017). DOI: 10.15252/embj.201695528
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For Immunization (frozen solution)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
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