HPLCを用いた神経伝達物質　依頼分析

エイコムでは、これまで蓄積してきたノウハウを使い電気化学検出器を主軸とした自社の分析機器を用いて分析​・サービスを行っています。お客様のニーズに応じアプリケーション、分析システムを選択し​高付加価値なサポートを致します。

• 脳関連の研究（うつ、アルツハイマー、パーキンソンなど）
• 血液および尿中の成分分析（癌マーカー、薬の血中濃度など）
• 筋肉や臓器から放出される成分分析
• 細胞から放出される成分分析（iPS細胞など）
• 抗酸化の研究（加齢、衛生など）

• 脳脊髄液　セロトニン
• 脳脊髄液　モノアミン
• 血中　カテコールアミン
• モノアミンおよび代謝物
• 脳ホモジ　アセチルコリン
• 血漿　NOx
• ビタミン C
• マイクロダイアリシス　アミノ酸
• マイクロダイアリシス　DL アラニン
• グリシン、グルタミン酸
• キサンツレン酸
• マイクロダイアリシス　GABA
• 細胞培養液　ドーパミン
• 脳ホモジ　メラトニン
• 脳ホモジ　セロトニン、ドーパミン
• 血清　MHPG
• 脊髄液　ノルアドレナリン、ドーパミン、セロトニン
• ニトロチロシン
• 皮下マイクロダイアリシス　ノルアドレナリン
• 血清　キヌレニン、セロトニン、トリプトファン
• 腎臓ホモジ　ノルアドレナリン
• 側坐核　ドーパミン

システム構成のイメージ

HPLC 機器を使用した　蛍光検出器、UV 検出器、電気化学検出器（ECD）で分析可能な物質であれば、ぜひご相談下さい。

電気化学検出器(ECD)※1

フローセル※2

マイクロダイアリシスとのコンビネーション実験

オープンフィールド

Morris水迷路、強制水泳

トレッドミル

高架式十字迷路

スキナー箱

その他いろいろ・・・
オプトジェネティクス
呼気ガスとの同時測定
薬物の同時投与

フリームービング回収　流路図（マイクロダイアリシス法）

脊髄：脊髄損傷の研究

筋肉：スポーツ科学（運動と生理）

皮下：塗薬がどれぐらい皮下へ浸透しているか

臓器（腸、心臓、唾液腺など）：口から投与した薬がどれぐらい末梢神経まで到達しているか
薬の影響で臓器内での物質がどれぐらい増減したか

脳組織
脳組織・ホモジネート
血清
血漿
尿
細胞培養液

• 1- Acetylcholine for Microdialysis
• 1 - 3 Acetylcholine and Choline for Tissue, Blood and Other Samples
• 2 - DA and 5-HT for Microdialysis
• 3 - Noradrenaline, Dopamine and Serotonin for Microdialysis
• 4 - Monoamines and Metabolites for Microdialysis
• 5 - 3 Monoamines & Metabolites for Tissue, Blood and Other Samples
• 6 - 3 DA & 5HT for Tissue, Blood and Other Samples
• 7 - 3 Catecholamine (NA, AD, DA) for Blood
• 8 - L-DOPA for Microdialysis
• 9 - Histamine(prelabel) for Microdialysis
• 10 - Amino Acids for Microdiaysis
• 10-3 Amino Acids for Tissue, Blood and Other Samples
• 11 - Glutamate and GABA for Microdialysis
• 12 - D-Amino Acid (D-Serine, L-Serine)
• 13 - Glutamate (Enzymatic) for Microdialysis
• 14 - Lactate (Enzymatic) for Microdialysis
• 15 - GSH and Thiols for Microdialysis
• 16 - GSH and GSSG(WE-DM) for Microdialysis
• 17 - 8-OH-dG for Microdialysis
• 18 - NOx (NO2-、NO3- ) for Microdialysis
• 19 - GABA for Microdialysis

2022

- Glucose infusion suppresses acute restraint stress-induced peripheral and central sympathetic responses in rats

- Investigating the Influence of GABA Neurons on Dopamine Neurons in the Ventral Tegmental Area Using Optogenetic Techniques

- The efficacy of duloxetine depends on spinal cholinergic plasticity in neuropathic pain model rats

- Behavioral and neurochemical effects of novel N-Benzyl-2-phenylethylamine derivatives in adult zebrafish

2021

- Spike firing attenuation of serotonin neurons in learned helplessness rats is reversed by ketamine

- l-Menthol increases extracellular dopamine and c-Fos-like immunoreactivity in the dorsal striatum, and promotes ambulatory activity in mice

- P2X2 receptors supply extracellular choline as a substrate for acetylcholine synthesis

- Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice

- The essential role of transcription factor Pitx3 in preventing mesodiencephalic dopaminergic neurodegeneration and maintaining neuronal subtype identities
during aging

- Ninjin'yoeito, a traditional Japanese medicine, increases dopamine content in PC12 cells

- Preventative Effects of 1-Methyl-1,2,3,4- Tetrahydroisoquinoline Derivatives (N-Functional Group Loading) On MPTP-Induced Parkinsonism In Mice

- Simultaneous serotonin and dopamine monitoring across timescales by rapid pulse voltammetry with partial least squares regression

- A novel in vitro assay model developed to measure both extracellular and intracellular acetylcholine levels for screening cholinergic agents

- Hormonal and Neurological Aspects of Dog Walking for Dog Owners and Pet Dogs

- Ninjin’yoeito, a traditional Japanese medicine, increases dopamine content in PC12 cells

- FFAR1/GPR40 Contributes to the Regulation of Striatal Monoamine Releases and Facilitation of Cocaine-Induced Locomotor Activity in Mice

- α-Synuclein-induced dysregulation of neuronal activity contributes to murine dopamine neuron vulnerability

- Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged
chronic unpredictable stress model

- Genetic dissection identifies Necdin as a driver gene in a mouse model of paternal 15q duplications

- Schizophrenia-Like Behavioral Impairments in Mice with Suppressed Expression of Piccolo in the Medial Prefrontal Cortex

- Vitamin B6 deficiency hyperactivates the noradrenergic system, leading to social deficits and cognitive impairment

- In Vivo Monitoring of Acetylcholine Release from Nerve Endings in Salivary Gland

- The Effects of Acute Glucoprivation on Adrenomedullary Function in SHR and WKY Rats

- Mice with mutations in Trpm1, a gene in the locus of 15q13.3 microdeletion syndrome, display pronounced hyperactivity and decreased anxiety-like behavior

- Activation of the cardiac non-neuronal cholinergic system prevents the development of diabetes-associated cardiovascular complications

- Dual actions of 5-MeO-DIPT at the serotonin transporter and serotonin 5-HT1A receptor in the mouse striatum and prefrontal cortex

- Decreased content of ascorbic acid (vitamin C) in the brain of knockout mouse models of Na+,K+-ATPase-related neurologic disorders

- Essential role for autophagy protein VMP1 in maintaining neuronal homeostasis and preventing axonal degeneration

- Non-neuronal cardiac acetylcholine system playing indispensable roles in cardiac homeostasis confers resiliency to the heart

- Beta-blockade Prevents Coronary Macro and Microvascular Dysfunction Induced by a High Salt Diet and Insulin Resistance in the Goto-Kakizaki Rat

- Selective inhibition of monoacylglycerol lipase is associated with passive coping behavior and attenuation of stress-induced dopamine release in the medial
prefrontal cortex

2020

- Mice with Mutations in Trpm1, a Gene within the locus of 15q13.3 Microdeletion Syndrome, Display Pronounced Hyperactivity and Decreased AnxietyLike
Behavior.

- Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

- Mice carrying a schizophrenia-associated mutation of the Arhgap10 gene are vulnerable to the effects of methamphetamine treatment on cognitive function:
association with morphological abnormalities in striatal neurons

- Noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in rats

- Absence of methamphetamine‐induced conditioned place preference in weaver mutant mice

- Monoamine and genome-wide DNA methylation investigation in behavioral addiction

- Highly Efficient Conversion of Motor Neuron-Like NSC-34 Cells into Functional Motor Neurons by Prostaglandin E2

- Monoamine and genome-wide DNA methylation investigation in behavioral addiction

- Gamma oryzanol impairs alcohol-induced anxiety-like behavior in mice via upregulation of central monoamines associated with Bdnf and Il-1β signaling

- Participation of the nucleus accumbens dopaminergic system in the antidepressant-like actions of a diet rich in omega-3 polyunsaturated fatty acids

- Early weaning augments the spontaneous release of dopamine in the amygdala but not the prefrontal cortex: An in vivo microdialysis study of male rats

- VGLUT2-expressing neurons in the vestibular nuclear complex mediate gravitational stress-induced hypothermia in mice

- Brain Angiopathy and Impaired Glucose Metabolism in Model Mice with Psychiatric-Related Phenotypes

- Tyr-Trp administration facilitates brain norepinephrine metabolism and ameliorates a short-term memory deficit in a mouse model of Alzheimer’s disease

- Twelve-Month Studies on Perilla Oil Intake in Japanese Adults—Possible Supplement for Mental Health​

- The Effect of Stressor Controllability on Social Hedonic Preference in Laboratory Rats

- Gamma Oryzanol Alleviates High-Fat Diet-Induced Anxiety-Like Behaviors Through Downregulation of Dopamine and Inflammation in the Amygdala of Mice

- Direct visualization of an antidepressant analog using surface-enhanced Raman scattering in the brain

- Prolyl-hydroxyproline, a collagen-derived dipeptide, enhances hippocampal cell proliferation, which leads to antidepressant-like effects in mice

- Protein Deficiency-Induced Behavioral Abnormalities and Neurotransmitter Loss in Aged Mice Are Ameliorated by Essential Amino Acids

- Angiopathy with impaired glucose logistics observed in schizophrenia-like model mice

- Trace Amine-Associated Receptor 5 Provides Olfactory Input Into Limbic Brain Areas and Modulates Emotional Behaviors and Serotonin Transmission

- Activation of mGluR5 and NMDA Receptor Pathways in the Rostral Ventrolateral Medulla as a Central Mechanism for Methamphetamine-Induced Pressor
Effect in Rats

2019

- (R)-Ketamine Induces a Greater Increase in Prefrontal 5-HT Release Than (S)-Ketamine and Ketamine Metabolites via an AMPA Receptor-Independent
Mechanism

- Kappa Opioid Receptors Drive a Tonic Aversive Component of Chronic Pain

- Parkinson’s disease recovery by GM1 oligosaccharide treatment in the B4galnt1+/− mouse model

- Hyperreflexia induced by XLR-11 smoke is caused by the pyrolytic degradant

2018

- Thiamine tetrahydrofurfuryl disulfide promotes voluntary activity through dopaminergic activation in the medial prefrontal cortex

- Effects of Memantine on Nitric Oxide Production and Hydroxyl Radical Metabolism during Cerebral Ischemia and Reperfusion in Mice

2017

- Overexpression of transmembrane protein 168 in the mouse nucleus accumbens induces anxiety and sensorimotor gating deficit

- Prenatal nicotine exposure decreases the release of dopamine in the medial frontal cortex and induces atomoxetine-responsive neurobehavioral deficits in mice

2016

- The Role of Spinal Dopaminergic Transmission in the Analgesic Effect of Nefopam on Rat Inflammatory Pain

- Abuse-Related Neurochemical Effects of Para-Substituted Methcathinone Analogs in Rats: Microdialysis Studies of Nucleus Accumbens Dopamine and
Serotonin

- Novel psychoactive benzofurans strongly increase extracellular serotonin level in mouse corpus striatum

- Sex Differences in Abuse-Related Neurochemical and Behavioral Effects of 3,4-methylenedioxymethamphetamine (MDMA) in Rats

- Brain regions and monoaminergic neurotransmitters that are involved in mouse ambulatory activity promoted by bupropion

2015

- Injection of corticotropin-releasing hormone into the amygdala aggravates visceral nociception and induces noradrenaline release in rats

2014

- Pregerminated Brown Rice Enhanced NMDA Receptor/CaMKIIα Signaling in the Frontal Cortex of Mice

- Monitoring Serotonin: Why Faster is Better

2013

- Physiologically Relevant Changes in Serotonin Resolved by Fast Microdialysis

- Pain-Related Depression of the Mesolimbic Dopamine System in Rats: Expression, Blockade by Analgesics, and Role of Endogenous κ-opioids

- Symmetrical Serotonin Release during Asymmetrical Slow-Wave Sleep: Implications for the Neurochemistry of Sleep–Waking States

2011

- Oral administration of glycine increases extracellular serotonin but not dopamine in the prefrontal cortex of rats

2009

- GABAergic transmission in the rat ventral pallidum mediates a saccharin palatability shift in conditioned taste aversion

2007

- Cortical Acetylcholine Release Is Lateralized during Asymmetrical Slow-Wave Sleep in Northern Fur Seals