Capping
In the process for phosphoramidite method, capping reaction after coupling reaction is important in order to avoid generating by-products. Before next step (oxidation) is carried out, unreacted nucleotide/oligonucleotide that is starting material at coupling reaction should be removed by the reaction. Because, by-products after oxidation step make a complicated for purification step when unreacted material remained.
Mechanism of capping step
Capping reaction using acetic anhydride
Product List
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Phosphoramidite-Type Capping Reagent
Acetyl-Type Capping Reagent (Acetonitrile solution)
Acetyl-Type Capping Reagent (THF solution)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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