Amyloid β ELISA Kits
The early stage of Alzheimer's disease is characterized by the accumulation of insoluble senile plaques formed by the aggregation and accumulation of amyloid β (Aβ). The accumulation of senile plaque is followed by neurofibrillary changes, sheddining of nerve cells, and consequent cognitive impairment in the period of few decades. Aβ has therefore been associated with the progression of Alzheimer's disease.
Our ELISA kits enable quantification of Aβ and high-molecular Aβ oligomers which are the aggregates of Aβ.
Features
- Can detect Aβ40 and 42 derived not only from tissue homogenates, cell culture supernates, cerebrospinal fluid (CSF), but also from plasma which is considered as hard to detect.
- Can detect high molecular Aβ oligomer (≧ 9 mer)
- Highly specific monoclonal antibody, developed by Takeda Pharmaceutical Company, Ltd..
- The antibodies used in the kits are commercially available. → For more information.
- Abundant references → See references list
Principle of the assay
Kit variation
| β Amyloid ELISA Kit Wako | To reduce unspecific bindings, using Fab' fragment antibody. |
|---|---|
| β Amyloid ELISA Kit Wako Ⅱ | To increase the stability of antigen-antibody reaction, using F(ab')2 fragment antibody. |
| β Amyloid ELISA Kit Wako, High Sensitive | The sensitivity is higher about ten times than the conventional products. Lower unspecific bindings by using Fab' fragment antibody. |
Selection Guide of Aβ-related ELISA kits
| Wako Cat. No. | Product Name | Clone No. of the antibodies | Detectable Aβ | Measuring Range (pmol/L) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Capture | Detection | Human Aβ (1-40) |
Human Aβ (1-42) |
Rat (Mouse) Aβ(1-40) |
Rat (Mouse) Aβ(1-42) |
Aβ (x-40) *1 |
Aβ (x-42) *1 |
Human Aβ Oligomer (≦8 mer) |
Human Aβ Oligomer (≧9 mer) |
|||
| 292-62301 | Human βAmyloid(1-40) ELISA Kit Wako |
BAN50 | BA27(Fab’) | + | - | - | - | - | - | *4 | *4 | 1.0 - 100 |
| 298-64601 | Human βAmyloid(1-40) ELISA Kit Wako Ⅱ*2 |
BAN50 | BA27(F[ab’]2) | + | - | - | - | - | - | *4 | *4 | 1.0 - 100 |
| 298-62401 | Human βAmyloid(1-42) ELISA Kit Wako |
BAN50 | BC05(Fab’) | - | + | - | - | - | - | *4 | *4 | 1.0 - 100 |
| 296-64401 | Human βAmyloid(1-42) ELISA Kit Wako , High-Sensitive |
BAN50 | BC05(Fab’) | - | + | - | - | - | - | *4 | *4 | 0.1- 20 |
| 294-62501 | Human／Rat βAmyloid(40) ELISA Kit Wako |
BNT77 | BA27(Fab’) | + | - | + | - | + | - | *4 | *4 | 1.0 - 100 |
| 294-64701 | Human／Rat βAmyloid(40) ELISA Kit Wako Ⅱ*3 |
BNT77 | BA27(F[ab’]2) | + | - | + | - | + | - | *4 | *4 | 1.0 - 100 |
| 290-62601 | Human／Rat βAmyloid(42) ELISA Kit Wako |
BNT77 | BC05(Fab’) | - | + | - | + | - | + | *4 | *4 | 1.0 - 100 |
| 292-64501 | Human／Rat βAmyloid(42) ELISA Kit Wako, High-Sensitive |
BNT77 | BC05(Fab’) | - | + | - | + | - | + | *4 | *4 | 1.0 - 20 |
| 298-80101 | High Molecular Amyloid β Oligomer ELISA Kit Wako |
BAN50 | BAN50 | - | - | - | - | - | - | - | + | 0.41 - 100*5 |
| 290-82001 | High Molecular Amyloid β Oligomer ELISA Kit Wako Ver.2 |
BAN50 | BAN50 | - | - | - | - | - | - | - | + | 0.16-40 (plasma, serum)*5 0.41 - 100 (CSF)*5 |
*1 Aβ(x-40) and Aβ(x-42) are Aβ peptides modified/ cleaved at N-terminal.
*2 Superior antigen-antibody reaction stability compared with 292-62301
*3 Superior antigen-antibody reaction stability compared with 294-62501
*4 Not tested.
*5 Calculated on the basis of 16-mer MAP peptide
Sample type
| Wako Cat. No. | Product Name | Cerebrospinal fluid | Brain tissue extract | Serum | Plasma | Cell culture supernatant |
|---|---|---|---|---|---|---|
| 292-62301 | Human βAmyloid(1-40) ELISA Kit Wako |
+ | + | + | + | + |
| 298-64601 | Human βAmyloid(1-40) ELISA Kit Wako Ⅱ*2 |
+ | + | + | + | + |
| 298-62401 | Human βAmyloid(1-42) ELISA Kit Wako |
+ | + | + | + | + |
| 296-64401 | Human βAmyloid(1-42) ELISA Kit Wako , High-Sensitive |
+ | + | + | + | + |
| 294-62501 | Human／Rat βAmyloid(40) ELISA Kit Wako |
+ | + | + | + | + |
| 294-64701 | Human／Rat βAmyloid(40) ELISA Kit Wako Ⅱ*3 |
+ | + | + | + | + |
| 290-62601 | Human／Rat βAmyloid(42) ELISA Kit Wako |
+ | + | + | + | + |
| 292-64501 | Human／Rat βAmyloid(42) ELISA Kit Wako, High-Sensitive |
+ | + | + | + | + |
| 298-80101 | High Molecular Amyloid β Oligomer ELISA Kit Wako |
+ | + | - | - | *4 |
| 290-82001 | High Molecular Amyloid β Oligomer ELISA Kit Wako Ver.2 |
+ | + | + | + | *4 |
*2 Superior antigen-antibody reaction stability compared with 292-62301
*3 Superior antigen-antibody reaction stability compared with 294-62501
*4 Not tested.
*5 Calculated on the basis of 16-mer MAP peptide
*6 Detectable, but the measured value tends to decrease over time compared to plasma samples.
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ELISA Kits
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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