Anti VGLUT Antibodies

The vesicular glutamate transporter (VGLUT) mediates the uptake of glutamate—the principal neurotransmitter in mammalian excitatory synaptic transmission—into synaptic vesicles. This process is thought to be driven by the proton electrochemical gradient. In mammals, three isoforms have been identified to date: VGLUT1, VGLUT2, and VGLUT3. These isoforms exhibit distinct expression patterns: VGLUT1 is mainly expressed in the cerebral cortex and hippocampus, whereas VGLUT2 is expressed in the thalamus, hypothalamus, and amygdala. Neurons expressing VGLUT3 are relatively rare, but mRNA expression studies have reported scattered presence in the striatum, hippocampus, cerebral cortex, and raphe nuclei¹⁾. In neuroscience research, anti-VGLUT antibodies are widely used as markers for glutamatergic neurons.

The “Anti VGLUT1, Guinea Pig” is a guinea pig polyclonal antibody, raised against VGLUT1. It can be used to perform multiplex immunohistochemistry.

Antibody Information

Application Data

Immunohistochemistry

Species:

Mouse

Site:

Hippocampus

Sample:

Frozen section

Antibody concentration:

1:1,600

[Note]
Neurons were stained with NeuroTrace™ 435/455 Blue Fluorescent Nissl Stain (Thermo Fisher Scientific, Inc.).

Data by courtesy of
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology

1. Gras, C. et al.: J. Neurosci., 22(13), 5442(2002).
   A third vesicular glutamate transporter expressed by cholinergic and serotoninergic neurons