Highly Cross-linked Polystyrene Support "Aminolinker PS"
The Highly Cross-linked Polystyrene Supports provides significant benefits for oligonucleotide synthesis. It delivers high coupling efficiency and excellent reagent washing efficiency during the synthesis process, resulting in oligonucleotides of higher purity compared to other supports. Additionally, its superior physical strength minimizes damage during synthesis. Its non-swelling property ensures minimal volume change throughout the process, eliminating the need to take swelling into consideration when packing the support into reaction vessels. As a result, it can be used as easily as controlled pore glass (CPG).
"Aminolinker PS" is a solid support specifically designed for the chemical synthesis of oligonucleotides. It features a porous, non-swelling polystyrene matrix. The physical characteristics of the polystyrene resin--such as particle size, shape, and pore size--are precisely controlled. This support is provided in its native form, functionalized with an amino linker. Nucleosides or universal linkers can be conjugated to it for oligonucleotide synthesis. Bulk quantities and custom modifications tailored to your specifications are also available. Please contact us for further details.
<Solid-phase Oligonucleotide Synthesis>
<Structure and Morphology of Aminolinker PS>
Structure of Aminolinker PS
SEM image of PS resin
Features
- Solid support for chemical synthesis of oligonucleotides
- Porous, non-swelling polystyrene support
- Precisely controlled physical characteristics such as particle size, shape, and pore size of the polystyrene resin
Physical Properties
| Aminolinker PS 1000A | |
|---|---|
| Particle size | 53～100 μm |
| Pore size | 1000Å (100 nm) |
| Dry volume | 3.9 mL/g |
| Swelling volume in acetonitrile | 4.1 mL/g |
| Swelling volume in toluene | 4.1 mL/g |
| Swelling volume in THF | － |
| Swelling volume in dichloromethane | － |
| Reactive group | NH2 |
| Amino Loading | 40-60 μmol/g |
Applications
Synthesis of Oligonucleotides (T10) Using Aminolinker PS 1000A in DMTr-Off Mode
a) PS 1000Å from FUJIFILM Wako Pure Chemical Industries
b) CPG 1000Å from other company
Figure HPLC analysis of ONs released from T10-loaded (a) UnyLinker™ PS 1000Å (R=Ph) and (b) UnyLinker™ CPG 1000Å (R=Me) under Basic conditions. Basic condition: AMA at 60℃ for 1 h.
HPLC_Condition of Oligonucleotide
| Column size | : | Wakopak® Ultra C18-2 φ2.1 mm×100 mm (D) |
| Mobile phase | : | A) 100 mmol/L TEAA aq., B) Acetonitrile |
| Gradient | : | 0-15 min B=5-15%, 15-20 min B=100%, 20-25 min B=5% |
| Flow rate | : | 0.3 mL/min |
| Temperature | : | 40℃ |
| Detection | : | UV260 nm |
| Injection vol. | : | 0.5 μL |
| Sample | : | DNA dT 10 mer, All PO |
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