Anti GFAP Antibodies

Glial fibrillary acidic protein (GFAP) is an intermediate filament protein with a molecular weight of approximately 50 kDa. It was initially isolated from brain lesions in patients with multiple sclerosis¹⁾. GFAP is widely recognized as a marker for astrocytes, as it is primarily expressed in these cells within the central nervous system. Additionally, GFAP expression has been reported in other cell types, such as neural stem cells in the hippocampus.

GFAP is thought to play a role in the structural organization of astrocytes. Previous studies have also shown that it is essential for the proliferation of reactive astrocytes and the formation of glial scars^2-3). Reactive astrocytes are cells that undergo morphological changes—such as process elongation and cellular hypertrophy—in response to central nervous system injury caused by trauma or neurodegenerative diseases. GFAP expression is known to increase under such pathological conditions compared to the normal state^2-3). Moreover, circulating GFAP has attracted attention as a biomarker that reflects neuroinflammatory states in neurodegenerative diseases, including Alzheimer’s disease.

Anti GFAP, Monoclonal Antibody(MO389) is a mouse monoclonal antibody against GFAP. It is suitable for astrocyte staining in both immunohistochemistry and immunocytochemistry.

Antibody Information

Clonality	Monoclonal
Antigen	Synthetic peptide (CQIRETpSLDTKS)
Host	Mouse
Formulation	PBS, 50% Glycerol
Conjugate	Unconjugated
Cross-reactivity	Human, Mouse
Application	Immunocytochemistry 1:1,000 Immunohistochemistry 1:500 Western Blotting 1:10,000

Application Data

Immunohistochemistry

Species: Mouse
Site: Thalamus
Sample: Frozen section
Antibody concentration: 1:500

Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1:500

Immunocytochemistry

Cell line: U251 (Human astrocytoma)
Antibody concentration: 1:1,000

1. Eng, L. et al.: Brain Res., 28(2), 351(1971).
   An acidic protein isolated from fibrous astrocytes
2. Sofroniew, M. V. and Vinters, H. V.: Acta Neuropathol., 119, 7(2010).
   Astrocytes: biology and pathology
3. Pekny, M. and Pekna, M.: J. Pathol., 204(4), 428(2004).
   Astrocyte intermediate filaments in CNS pathologies and regeneration