Exosomes, from Mesenchymal Stem Cells, Purified
In extracellular vesicle (EV) experiments, including those involving exosomes, EVs with verified marker protein expression serve as a positive control to confirm whether the experiment has been performed successfully. Fujifilm Wako offers EVs isolated and purified from mesenchymal stem cell/mesenchymal stromal cell (MSC) culture supernatant using the PS affinity method.
Product Overview
This product consists of highly pure, intact extracellular vesicles (EVs) isolated and purified from bone marrow-derived MSC (BMMSC) culture supernatant using the PS affinity method. In addition to the standard EV markers CD9, CD63, and CD81, positive expression of CD59 and CD73 — well-established markers of MSC-derived EVs — has also been confirmed. The product is suitable for use as a positive control or reference standard in EV research.
Features
- High purity
- High stability
- Non-lyophilized
- EV marker proteins (CD9, CD63, CD81) positive
- MSC-derived EV marker proteins (CD59, CD73) positive
Expression of CD59 and CD73 has been confirmed only by Western blotting.
Specification
Properties: Solution
Formulation: EVs, TBS, EDTA, Polymer (stabilizer)
Protein concentration*: 10 μg/mL
* The protein concentration was calculated from the signal values of the EV marker protein (CD63).
Data
Particle Analysis by NTA
Particle analysis was carried out on this product using Nanoparticle Tracking Analysis (NTA) with a NanoSight instrument.
[Result]
This product provided a high degree of uniformity in particle size.
MSC-derived EV Marker Expression
Expression of the MSC-derived EV markers CD59 and CD73, along with the EV marker CD63, was assessed in this product by Western blotting.
[Result]
Expression of the MSC-derived EV markers CD59 and CD73, and the EV marker CD63, was confirmed by Western blotting under non-reducing conditions.
M : Marker
1 : Sample (non-reducing condition)
2 : Sample (reducing condition)
Evaluation of Anti-inflammatory Activity
This product was added to cultured monocytes stimulated with 0.25 EU/mL LPS, and the anti-inflammatory activity (suppression of NF-κB signaling) was evaluated using the LumiMAT™ Pyrogen Detection Kit, a pyrogen detection system utilizing NF-κB signaling as a readout.
[Result]
This product exhibited concentration-dependent anti-inflammatory activity.
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Exosomes, from Mesenchymal Stem Cells, Purified
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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