TLC Plate (Polyamide)
Thin layer chromatography (TLC) is a method that uses a thin layer made of a stationary phase such as silica gel, polyamide, alumina, etc. on a glass or aluminum plate, separates the mixture sample into each component with a mobile phase. TLC is widely used for substance identification, purity testing, and for examining separation conditions when using column chromatography. Fujifilm Wako provides Silica Gel TLC Plates (Unmodified), NH2 modified Silica Gel TLC Plates, and TLC plates, which thin layer is made from polyamide.
Polyamide FM Plates are TLC plates that use polyamide as a carrier and add three types of fluorescent substances mixture (red, green, and blue). Polyamides are used in adsorption chromatography mode and are suitable for the separation of phenols and carboxylic acids, making them ideal for the analysis of preservatives, fungicides, antioxidants, sweeteners, and colorants in foods. In addition, due to the addition of three types of fluorescent substances mixture, the Polyamide FM Plate shows a substance-specific ultraviolet absorption when irradiated with a wide range of ultraviolet rays (250 to 400 nm), so the target substance can be observed as a colored spot such as red or blue.
How to Choose a TLC Plate
(1) Type of carrier
| Unmodified silica gel | Used in adsorption chromatography mode, mainly suitable for separation of acidic or neutral compound. |
|---|---|
| Chemically modified silica gel | Various functional groups are bonded to the silanol groups on the surface of unmodified silica gel to change the function. C18 modified silica gel, which is octadecyl group (ODS) bonded silica gel, suitable for reversed-phase chromatography mode. NH2 modified silica gel, which is aminopropyl group bonded silica gel, suitable for separation of sugars, catecholamines, etc. |
| Polyamides | Used in adsorption chromatography mode, suitable for separation of phenols and carboxylic acids. |
(2) Pore size/specific surface area
The smaller the pore size, the greater the specific surface area of the silica gel and the higher the resolution of small molecules. Conversely for macromolecules such as proteins, the larger the pore size, the higher the resolution. It is necessary to select an appropriate pore size so that sample molecules can easily enter and exit the pores.
(3) Type of support
Glass has excellent chemical resistance and is the most widely used. Aluminum and paper can be cut easily with scissors, but the carrier peels off from support or the TLC bends depending on the developing solvent. In addition, strong acids/bases cannot be used as developing solvents or coloring reagents.
(4) Type of fluorescent substance
| Not contain | It is suitable for detection of fluorescing samples and spot detection using coloring TLC stains (sulfuric acid, ninhydrin, iodine, etc.) because it does not contain fluorescent substances. |
|---|---|
| Mono fluorescent substance | 254 nm UV irradiation causes the entire plate to fluoresce green, and samples with UV absorption turn black colored spots. |
| Mixture fluorescent substance | Red, green, and blue fluorophores substance are added. Under broadband UV (250-400 nm) irradiation causes the entire plate to white, and samples with UV absorption turn the characteristic colored spot such as, red, blue, etc. |
Physical Properties of TLC Plates
| Product name | Pore size | Specific surface area | Pore volume | Layer thickness | Support | Sticking agent | Fluorescent substance | Detection |
|---|---|---|---|---|---|---|---|---|
| Polyamide FM Plate | - | - | - | 70-110 μm | Glass plate | Cast | Mixture fluorescent substance | Broad UV (250-400 nm) |
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