Analysis for PFAS (PFOS, PFOA, PFHxS, etc.)

Fujifilm Wako provides mixture standard solutions and internal standard solution (¹³C labels) for PFHxS, PFOS, and PFOA which are compounds subject to JP Water Quality Act. Both are mixture standard solutions with clear concentration value of linear PFAS.

Features

✔ Mixture standard solutions with clear concentration value of linear PFAS!

LC/MS Chromatogram (3 PFCs Mixture Standard Solution)

Analysis conditions

[HPLC]

Column

： Wakopak Ultra C18-2　2.1×100 mm

Delay column

： Wakopak Ultra C18-3　2.0×150 mm

Column temperature

： 40℃

Eluent

： A) 0.01 mol/L CH₃COONH₄ in H₂O, B) CH₃CN

Gradient

：

Time (min)	B conc. (%)
0-2	20
2-22	20-95
22-30	95

Flow rate

： 0.2 mL/min.

[MS]

Ionization

： ESI

Mode

： SIM

Features

• Guarantees PFOA, PFOS, and PFHxS in the solvent is 1 ng/L or less.
• Suitable for pretreatment and instrument cleaning.
• Guarantees HPLC specification items.

Example Test Items (Methanol)

We conducted a blank test using a 1000-fold concentrate of this product and confirmed that PFOA, PFHxS, and PFOS were at low levels.

Ultrapure Water

Methanol

Acetonitrile

Features

• SPE columns utilizing a filler with both reverse-phase and anion-exchange properties.
• Two types are available. Cartridge-type and syringe-type.
• Capable of high-efficiency recovery of 10 types of PFAS, including PFOS, PFOA, and PFHxS.

SPE Operation Examples*

Cartridge-type

Syringe-type

Column Conditioning
1) 0.1%NH₃ in CH₃OH 4 mL
2) CH₃OH 4 mL
3) H₂O 4 mL

Loading
Water + Internal standard solution and Standard solution
*Flow rate: 5 mL/min
Washing
H₂O (15 mL)

Dry (N₂ purge)

Extraction
0.1% NH₃ in CH₃OH (5 mL)
*Elution in the opposite direction to the loading direction

Extraction
0.1% NH₃ in CH₃OH (5 mL)

Concentration (→0.5 mL)

LC/MS/MS
Injection volume: 5 μL

*Conditions may need to be reviewed.

Additive Recovery Test for 10 PFAS*

*This data is an analysis example and does not guarantee the product.

Additive recovery test (PFOA, PFOS) using Presep^®-C Agri (Short)

Sample

Ultrapure Water: for PFAS Analysis
Standard Solution: PFOS・PFOA mixture standard solution (PFOS (linear): 78 μg/L, PFOA (linear): 100 μg/L)
Internal Standard Solution: ¹³C-PFOS・¹³C-PFOA mixture standard solution (PFOS: 100 μg/L, PFOA: 100 μg/L)

• Collection / Concentration Operation

  Water (500 mL) + Standard and Internal Standard Solution

  ▼

  Presep®-C Agri (Flow rate 5 mL/min.)

  ▼

  Washing (H2O 5 mL)

  ▼

  Dry (N2 purge)

  ▼

  Extraction (0.1%NH3 in CH3OH 5 mL)

  ▼

  Concentration (→0.5 mL)

  ▼

  LC/MS/MS

• Column Conditioning

  1. 0.1%NH₃ in CH₃OH 4 mL
  2. CH₃OH 4 mL
  3. H₂O 4 mL

LC/MS/MS Conditions

• Column: Wakopak^® Ultra C18-3 2.0 mm×150 mm
  Eluent: A)10 mM CH₃COONH₄ in H₂O, B)CH₃CN
  LC/MS/MS: Prominence LC-20ADXR / LCMS 8030 plus

• Gradient:

  Time (min)	B conc. (%)
  0-1	25
  1-26	25-100
  26-30	100
  30-30.01	100-25
  30.01-35	25

Result

Chromatogram

Recovery Result

	Monitoring Ions (m/z)		Recovery Rate
	Precursor	Product
PFOS	498.8	80.0	100.9%
PFOA	412.9	169.1	95.0%