Anti Sbp2l Antibodies
Sbp2l (SECIS-binding protein 2-like) is a RNA-binding protein expressed specifically in oligodendrocytes. It is considered essential for the normal maturation of oligodendrocytes. Immunohistochemical analysis has confirmed that Sbp2l colocalizes with known oligodendrocyte markers such as Olig2 and MBP, supporting its utility as an oligodendrocyte marker.
Fujifilm Wako offers a polyclonal antibody against Sbp2l raised in rabbits. It can be used for immunohistochemistry of oligodendrocytes and for Western blotting.
What is Sbp2l?
Sbp2l (SECIS-binding protein 2-like), also known as Secisbp2L, is an RNA-binding protein. Sbp2l is an ortholog of Sbp2 (Secisbp2), a well-characterized RNA-binding protein that binds the Sec insertion sequence (SECIS) motif in the 3′-UTR of selenoprotein mRNAs. Like Sbp2, Sbp2l binds SECIS-like structures in the 3′-UTR of mRNAs1).
Integrative transcriptomic analysis identified Sbp2l as an RNA-binding protein expressed specifically in oligodendrocytes1). Because oligodendrocyte maturation is impaired in Sbp2l-knockout mice, Sbp2l is considered essential for the normal maturation of these cells. Furthermore, immunohistochemical analysis has confirmed that Sbp2l colocalizes with known oligodendrocyte markers such as Olig2 and MBP1), supporting its utility as an oligodendrocyte marker.
Anti Sbp2l, Rabbit
“Anti Sbp2l, Rabbit” is a polyclonal antibody against Sbp2l raised in rabbits1). It can be used for immunohistochemistry of oligodendrocytes and for Western blotting.
Antibody Information
| Clonality | Polyclonal |
|---|---|
| Antigen | Synthetic peptide (C-terminus of mouse Sbp2l, 1021–1089 aa) |
| Host | Rabbit |
| Formulation | D-PBS (-) |
| Conjugate | Unconjugated |
| Cross-reactivity | Mouse |
| Application | Immunohistochemistry (Frozen section) 1:500 Western blotting 1:1,000 |
Application Data
Immunohistochemistry
>Immunohistochemstry of Sbp2l-knockout mice
- Species
- Mouse
- Site
- Spinal cord
- Sample
- Frozen section
- Antibody concentration
- 1:500
[Result]
In the immunohistochemstry of Sbp2l-knockout mice using this product, almost no Sbp2l signal was observed, confirming that this antibody shows high specificity for mouse Sbp2l.
>Double staining with oligodendrocyte markers
- Species
- Mouse
- Site
- Spinal cord
- Sample
- Frozen section
- Antibody concentration
- 1:500
- Note
- Arrowheads represent Sbp2l/MBP-positive cells
![[免疫組織染色]オリゴデンドロサイトマーカーとの共染色](images/03605_img04.png)
- Species
- Mouse
- Site
- Spinal cord
- Sample
- Frozen section
- Antibody concentration
- 1:500
![[免疫組織染色]オリゴデンドロサイトマーカーとの共染色](images/03605_img05.png)
- Species
- Mouse
- Site
- Spinal cord
- Sample
- Frozen section
- Antibody concentration
- 1:500
- Note
- Arrowheads represent Sbp2l/Olig2-positive cells
[Result]
Sbp2l was confirmed to be expressed in the same cells as the known oligodendrocyte markers MBP, Qki7, and Olig2.
>Double staining with oligodendrocyte precursor cell markers
- Species
- Mouse
- Site
- Spinal cord
- Sample
- Frozen section
- Antibody concentration
- 1:500
- Note
- Arrows represent Sbp2l-positive cells
(Sbp2l showed almost no colocalization with PDGFRα)
[Result]
Sbp2l showed almost no colocalization with PDGFRα, a known marker of oligodendrocyte precursor cells.
>Double staining with astrocyte markers
- Species
- Mouse
- Site
- Spinal cord
- Sample
- Frozen section
- Antibody concentration
- 1:500
- Species
- Mouse
- Site
- Spinal cord
- Sample
- Frozen section
- Antibody concentration
- 1:500
[Result]
Sbp2l showed almost no colocalization with the known astrocyte markers GFAP and glutamine synthetase (GS).
Western Blotting
>Sbp2l Knockdown by Sbp2l-specific siRNA
Cell: Oligodendrocyte precursor cells
Antibody concentration: 1:1,000
[Result]
The Sbp2l signal decreased following knockdown by Sbp2l-specific siRNA.
The data for immunohistochemistry and Western blotting are reproduced from reference 1) under CCBY 4.0.
References
- Yugami, M. et al.: iScience, 26(12), (2023).
Sbp2l contributes to oligodendrocyte maturation through translational control in Tcf7l2 signaling
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Anti Sbp2l, Rabbit (polyclonal antibody)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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